Osteogenic cell response to calcium aluminate-based cement

Author(s)

Castro-Raucci, L. M. S. ; Teixeira, L. N. ; Oliveira, I. R. ; Raucci-Neto, W. ; Jacobovitz, M. ; Rosa, A. L. ; de Oliveira, P. T.

• Document type: Journal article
• Source: International Endodontic Journal; v. 50, n. 8, p. 771-779, AUG 2017.
• Web of Science Citations: 5

Abstract

AimTo evaluate the effect of a calcium aluminate-based cement (CAC+) on the development of the osteogenic phenotype invitro. MethodologyRat calvaria-derived cells were grown on Thermanox((R)) coverslips for 24h and then exposed to either samples (4-h set) of CAC+ or mineral trioxide aggregate (MTA) placed on Transwell((R)) inserts for periods of up to 14days. Nonexposed cultures were used as the controls. The comparisons were made using the nonparametric Kruskal-Wallis test, followed by the Student-Newman-Keuls post hoc test when appropriate. ResultsThe results showed that proximity to MTA or CAC+ samples inhibited cell growth, whereas at a distance, viable and proliferative cells adhered to and spread on the Thermanox((R)), expressing osteoblast differentiation markers prior to mineralization of the extracellular matrix. Compared with MTA, the osteogenic cell cultures exposed to CAC+ exhibited significantly greater cell viability, alkaline phosphatase (ALP) activity and expression of runt-related transcription factor 2, osterix, ALP, bone sialoprotein and osteocalcin (P<0.05 for all). For the osteogenic cell cultures exposed to CAC+, the quantification of matrix mineralization was not altered (P>0.05). ConclusionsCAC+ supported the acquisition of the osteogenic cell phenotype invitro, rendering this novel material a potential alternative to MTA in endodontic procedures. Further invivo studies are needed to verify if the beneficial invitro effects of CAC+ on osteoblastic cells correspond to an increase and/or acceleration of bone repair in the periapical region. (AU)

• FAPESP's process: 10/50936-4 - The effects of aluminate cement preparations on the progression of osteogenic cell cultures and on undifferentiated mesenchymal pulp cells
• Grantee: Paulo Tambasco de Oliveira
• Support Opportunities: Regular Research Grants