Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Preclinical study of a cost-effective photodynamic therapy protocol for treating oral candidoses

Full text
Author(s):
da Silva, Nathalia Ramos ; Ribeiro, Daniela Garcia ; Mardegan Issa, Joao Paulo ; Bonfa, Karla ; Menezes, Michelli Sobreiro ; Oliveira, Viviane de Cassia ; de Souza, Raphael Freitas
Total Authors: 7
Document type: Journal article
Source: Lasers in Medical Science; v. 32, n. 6, p. 1253-1260, AUG 2017.
Web of Science Citations: 2
Abstract

Photodynamic therapy (PDT) is a promising treatment for oral candidoses. Its use as an alternative to antifungals prevents several adverse effects, including microbial resistance. However, most PDT protocols do not employ devices and consumables commonly available in dental practice, thus influencing treatment affordability. This study aimed to determine the efficacy of a PDT method based on light curing units' blue LEDs combined to a plaque-disclosing composition (5% erythrosine) against C. albicans in culture and in a murine model of oral candidosis. Standard and resistant fungal strains were tested in vitro in planktonic and biofilm forms. PDT (pre-irradiation time periods: 30 and 60 s; irradiation time: 3 min) was compared to control conditions without light and/or erythrosine. Mice with induced oral candidosis (n = 40) randomly received PDT or similar control conditions with subsequent C. albicans count. These mice underwent histological analysis, as well as 12 healthy mice submitted to experimental treatments. PDT completely inactivated C. albicans planktonic cells and biofilm. Control conditions presented minor differences (ANOVA, p < 0.05), with mean values ranging from 5.2 to 6.8 log10 (UFC/mL). Infected mice presented no significant difference in C. albicans counts consequent to treatments (ANOVA, p = 0.721), although the PDT protocol was able to enhance the inflammatory infiltrate in healthy mice. It can be concluded that the tested PDT protocol can inactivate C. albicans but still needs further investigation in order to achieve efficacy and safety. (AU)

FAPESP's process: 12/02206-2 - Preclinical study of a cost-effective photodynamic therapy protocol for treating oral candidoses
Grantee:Raphael Freitas de Souza
Support type: Regular Research Grants
FAPESP's process: 12/22026-9 - Preclinical study of a cost-effective photodynamic therapy protocol for treating oral candidoses
Grantee:Nathalia Ramos da Silva
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/04179-5 - Efficacy of a photodynamic therapy protocol for the inactivation of Candida species
Grantee:Karla Bonfa
Support type: Scholarships in Brazil - Scientific Initiation