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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Impact of light quality on flavonoid production and growth of Hyptis marrubioides seedlings cultivated in vitro

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Author(s):
Pedroso, Rita Cassia N. ; Branquinho, Nubia Angelica A. ; Hara, Alessandra C. B. A. M. ; Costa, Alan Carlos ; Silva, Fabiano Guimaraes ; Pimenta, Leticia P. ; Silva, Marcio Luiz A. ; Cunha, Wilson Roberto ; Pauletti, Patricia M. ; Januario, Ana Helena
Total Authors: 10
Document type: Journal article
Source: REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY; v. 27, n. 4, p. 466-470, JUL-AUG 2017.
Web of Science Citations: 11
Abstract

ABSTRACT Hyptis marrubioides Epling, Lamiaceae, a species from Brazilian Cerrado, has been used against gastrointestinal infections, skin infections, pain, and cramps. Herein, H. marrubioides seedlings were cultured in vitro under different wavelengths (white, blue, green, red, and yellow) with 50 µmol m-2 s-1 irradiance and a 16-h photoperiod. After 20 and 30 days of cultivation, shoot length, leaf number, fresh mass, and dry mass were evaluated. The flavonoid rutin content was determined by the HPLC-DAD method. The shoots were longer in plants cultivated under yellow (16.603 ± 0.790 cm, 1.8-fold), red (15.465 ± 0.461 cm, 1.7-fold), and green (14.677 ± 0.737 cm, 1.6-fold) lights than in control plants exposed to white light (9.203 ± 0.388 cm). The number of leaves increased in plants exposed to red (23.425 ± 1.138, 1.1-fold) and green (22.725 ± 1.814, 1.1-fold) lights, compared to control plants (20.133 ± 0.827). Fresh (0.665 ± 0.048 g, 1.2-fold) and dry (0.066 ± 0.005 g, 1.3-fold) mass of seedlings were the highest in seedlings grown under red light, compared to seedlings grown under white light (0.553 ± 0.048 and 0.028 ± 0.004, respectively). However, rutin production was higher under white (0.308 mg g-1 of dry weight) and blue lights (0.298 mg g-1 of dry weight). Thus, red light induces plant growth and increases leaf number and dry weight in in vitro-cultivated H. marrubioides, whereas blue and white lights promote the greatest rutin accumulation. (AU)

FAPESP's process: 11/00631-5 - Bioactive compounds from medicinal and food plants of the cerrado and Pantanal and their endophytic microbiota
Grantee:Ana Helena Januário
Support Opportunities: Regular Research Grants