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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Absorbance detector for high performance liquid chromatography based on a deep-UV light-emitting diode at 235 nm

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Petruci, Joao Flavio da Silveira ; Liebetanz, Michael G. ; Cardoso, Arnaldo Alves ; Hauser, Peter C.
Total Authors: 4
Document type: Journal article
Source: Journal of Chromatography A; v. 1512, p. 143-146, AUG 25 2017.
Web of Science Citations: 2

In this communication, we describe a flow-through optical absorption detector for HPLC using for the first time a deep-UV light-emitting diode with an emission band at 235 nm as light source. The detector is also comprised of a UV-sensitive photodiode positioned to enable measurement of radiation through a flow-through cuvette with round aperture of 1 mm diameter and optical path length of 10 mm, and a second one positioned as reference photodiode; a beam splitter and a power supply. The absorbance was measured and related to the analyte concentration by emulating the Lambert-Beer law with a log-ratio amplifier circuitry. This detector showed noise levels of 0.30 mAU, which is comparable with our previous LED-based detectors employing LEDs at 280 and 255 nm. The detector was coupled to a HPLC system and successfully evaluated for the determination of the anti-diabetic drugs pioglitazone and glimepiride in an isocratic separation and the benzodiazepines flurazepam, oxazepam and clobazam in a gradient elution. Good linearities (r>0.99), a precision better than 0.85% and limits of detection at sub-ppm levels were achieved. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 16/16830-0 - Miniaturized platform for gaseous sensing using LED's and microfluidic paper analytical devices
Grantee:João Flávio da Silveira Petruci
Support type: Scholarships abroad - Research Internship - Post-doctor