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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Prevalence of Helicobacter pylori vacA, cagA, dupA and oipA Genotypes in Patients with Gastric Disease

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Author(s):
Sallas, Mayara Luciana ; Melchiades, Jessica Lima ; Zabaglia, Luanna Munhoz ; do Prado Moreno, Juliana Ribeiro ; Orcini, Wilson Aparecido ; Chen, Elizabeth Suchi ; Cardoso Smith, Marilia de Arruda ; Marques Payao, Spencer Luiz ; Rasmussen, Lucas Trevizani
Total Authors: 9
Document type: Journal article
Source: ADVANCES IN MICROBIOLOGY; v. 7, n. 1, p. 1-9, JAN 2017.
Web of Science Citations: 2
Abstract

Gastric diseases such as chronic gastritis and gastric cancer are most commonly caused by virulence factors of Helicobacter pylori (H. pylori), such as the vacA, cagA, dupA and oipA genes. Therefore, this study investigated the prevalence and the combination of these virulence factors from patients with gastric diseases. The endoscopic biopsies were obtained from 516 patients with gastric symptoms, 101 of which were from patients with normal gastric tissue, 365 of which were from patients with chronic gastritis, and 50 of which were from patients with gastric cancer. H. py-lori and the virulence factors were detected by PCR. The oipA gene exhibited an increased risk for chronic gastritis (p = 0.0296), and the vacA gene demonstrated a risk for gastric cancer from chronic gastritis (p = 0.0002). Based on the combination of the virulence factors, cagA, vacA, dupA and oipA genes exhibited a high prevalence in patients with chronic gastritis and gastric cancer. The cagA+/dupA+ genotype demonstrated a significant correlation in patients with normal gastric mucosa (p = 0.0278). In the chronic gastritis group, a significant association was observed between the cagA+ and the vacA s1m1 genotypes (p < 0.0001), the cagA+/dupA+ genotypes (p = 0.0183), the dupA+/oipA+ genotypes (p < 0.0001), and the du-pA+/vacA s1m1 genotypes (p = 0.0008) genotypes. This study revealed a high prevalence of the combination of cagA, vacA, dupA, and oipA genes, which contributed to the risk of developing gastroduodenal diseases. Furthermore, this is the first study to reveal a high prevalence of the oipA gene in H. pylori isolates in Brazil. (AU)

FAPESP's process: 15/15253-7 - Detection of virulence marker of h. pylori, oipA gene, polymorphism characterization + 144 and analysis of gene expression of interleukin 2 in inflamatory gastric disease and gastric cancer
Grantee:Mayara Luciana Sallas
Support type: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 15/11371-5 - Analysis of expression of Micro RNAs and genes associated with apoptosis and chronic inflammation in gastric diseases: a possible relation with Helicobacter pylori
Grantee:Lucas Trevizani Rasmussen
Support type: Regular Research Grants