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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Amphotericin B as an inducer of griseofulvin-containing guttate in the endophytic fungus Xylaria cubensis FLe9

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Author(s):
Caraballo-Rodriguez, Andres M. ; Mayor, Cassia A. ; Chagas, Fernanda O. ; Pupo, Monica T.
Total Authors: 4
Document type: Journal article
Source: Chemoecology; v. 27, n. 5, p. 177-185, OCT 2017.
Web of Science Citations: 2
Abstract

Microorganisms interact with each other via metabolic exchange. Several studies have revealed that microbial metabolites may act as mediators of microbial interactions. During our previous work with endophytes isolated from the Brazilian medicinal plant Lychnophora ericoides, we demonstrated that the well-known antifungal compound amphotericin B, produced by the endophytic actinobacterium Streptomyces albospinus RLe7, may trigger chemical responses in endophytic fungi. In this study, we cultured endophytic fungi in amphotericin B-enriched media to verify whether other chemical responses could be induced. Interestingly, one fungal strain showed a differential response under the tested conditions. When the fungus Xylaria cubensis FLe9 was cultured in amphotericin B-enriched media, a mycelial guttate was observed. Investigation of the fungal extracts from X. cubensis FLe9 and purification of fungal metabolites were performed using high-performance liquid chromatography coupled to a diode array detector. Isolated compounds were characterized on the basis of nuclear magnetic resonance and mass spectrometry data. Therefore, we report on the overproduction of the fungal metabolites griseofulvin (1) and dechlorogriseofulvin (2) when the endophytic fungus Xylaria cubensis FLe9 was exposed to amphotericin B. Both fungal compounds 1 and 2 were also detected in the mycelial guttate produced when X. cubensis FLe9 was exposed to amphotericin B. Since the amphotericin B-producing actinobacterium S. albospinus RLe7 and the griseofulvin-producing fungus X. cubensis FLe9 are endophytes from the same host, biosynthetic induction of fungal compounds may suggest a potential signaling role for amphotericin B in natural environments. However, this hypothesis needs to be further investigated in field experiments. (AU)

FAPESP's process: 14/01651-8 - Elicitation of natural products biosynthesis from endophytic microorganisms' interactions
Grantee:Andrés Mauricio Caraballo Rodríguez
Support type: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 12/21803-1 - Elicitation of Natural Products biosintesis by endophytic microorganisms interaction
Grantee:Andrés Mauricio Caraballo Rodríguez
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 08/09540-0 - New strategies in the search of natural antibiotics: symbiotic microorganisms as sources of compounds and in vivo assay based on Caenorhabditis elegans infection model
Grantee:Mônica Tallarico Pupo
Support type: Regular Research Grants