Pseudomonas aestus sp nov., a plant growth-promoting bacterium isolated from mangrove sediments

Author(s)

Vasconcellos, Rafael L. F. ; Santos, Suikinai Nobre ; Zucchi, Tiago Domingues ; Silva, Fabio Sergio Paulino ; Souza, Danilo Tosta ; Melo, Itamar Soares

• Document type: Journal article
• Source: Archives of Microbiology; v. 199, n. 8, p. 1223-1229, OCT 2017.
• Web of Science Citations: 1

Abstract

Strain CMAA 1215(T), a Gram-reaction-negative, aerobic, catalase positive, polarly flagellated, motile, rod-shaped (0.5-0.8 x 1.3-1.9 A mu m) bacterium, was isolated from mangrove sediments, Canan,ia Island, Brazil. Analysis of the 16S rRNA gene sequences showed that strain CMAA 1215(T) forms a distinct phyletic line within the Pseudomonas putida subclade, being closely related to P. plecoglossicida ATCC 700383(T), P. monteilii NBRC 103158(T), and P. taiwanensis BCRC 17751(T) of sequence similarity of 98.86, 98.73, and 98.71%, respectively. Genomic comparisons of the strain CMAA 1215(T) with its closest phylogenetic type strains using average nucleotide index (ANI) and DNA:DNA relatedness approaches revealed 84.3-85.3% and 56.0-63.0%, respectively. A multilocus sequence analysis (MLSA) performed concatenating 16S rRNA, gyrB and rpoB gene sequences from the novel species was related with Pseudomonas putida subcluster and formed a new phylogenetic lineage. The phenotypic, physiological, biochemical, and genetic characteristics support the assignment of CMAA 1215(T) to the genus Pseudomonas, representing a novel species. The name Pseudomonas aestus sp.nov. is proposed, with CMAA 1215(T) (=NRRL B-653100(T) = CBMAI 1962(T)) as the type strain. (AU)

• FAPESP's process: 13/16037-0 - Isolation and characterization of genes correlated to biosynthesis of antitumor molecules in Streptomyces sp.
• Grantee: Suikinai Nobre Santos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 12/16623-4 - Study of Pseudomonas putida (strain 103) genome, genes of salt resistance identification and heterologous expression of phosphate solubilization pqq gene
• Grantee: Rafael Leandro de Figueiredo Vasconcellos
• Support Opportunities: Scholarships in Brazil - Post-Doctoral