| Full text | |
| Author(s): |
Assoni, Amanda D.
;
Amorim, Alessandro B.
;
Saleh, Mayra A. D.
;
Tse, Marcos L. P.
;
Berto, Dirlei A.
Total Authors: 5
|
| Document type: | Journal article |
| Source: | ANIMAL NUTRITION; v. 3, n. 3, p. 225-231, SEP 2017. |
| Web of Science Citations: | 2 |
| Abstract | |
The use of stable isotope analysis as a tool for characterization of carbon turnover (delta C-13) in piglet's tissues by tracing its feeding system has drawn attention. Thus, this study aimed at evaluating the influence of dietary glutamine, glutamic acid and nucleotides supplementation on carbon turnover in fundic-stomach region of weaned piglets at an average age of 21 days. The diets consisted of additive-free diet - control (C); 1% glutamine (G); 1% glutamic acid (GA) and 1% nucleotides (Nu). At weaning day (day 0: baseline), 3 piglets were slaughtered to quantify the delta C-13 of stomach. The remaining 120 piglets were blocked by weight and sex, randomly assigned to pens with 3 piglets slaughtered per treatment at days 1, 2, 4, 5, 7, 9, 13, 20, 27 and 49 after weaning in order to verify the fundic-stomach isotopic composition by treatments. Samples were analyzed in terms of C-13/C-12 ratio by mass spectrometry and converted to relative isotopic enrichment values (delta C-13 parts per thousand) used to plot the first order exponential curves over time using OriginPro 8.0 software. The inclusion of glutamine, glutamate and nucleotides in piglet's diets has accelerated the carbon turnover in stomach during the post-weaning period, demonstrating also that glutamate has guaranteed fastest C-13 incorporation rate on fundic-stomach region and pH-lowering. Besides that, stable isotopes technique (delta C-13) has proved to be an important methodology to determine the time-scales at which piglets shift among diets with different isotopic values, characterizing the trophic effects of additives and the phenotypic flexibility of stomach. (C) 2017, Chinese Association of Animal Science and Veterinary Medicine. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). (AU) | |
| FAPESP's process: | 14/13518-0 - Carbon turnover of stomach of weaned piglets fed with glutamate, glutamine and nucleotides in the diet |
| Grantee: | Amanda Dias Assoni |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |