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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Head and neck radiotherapy does not increase gelatinase (metalloproteinase-2 and-9) expression or activity in teeth irradiated in vivo

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Author(s):
Gomes-Silva, Wagner ; Prado Ribeiro, Ana Carolina ; de Castro Junior, Gilberto ; Salvajoli, Joao Victor ; Palmier, Natalia Rangel ; Lopes, Marcio Ajudarte ; Rocha, Marcelo Marques ; de Goes, Mario Fernando ; Brandao, Thais Bianca ; Santos-Silva, Alan Roger
Total Authors: 10
Document type: Journal article
Source: ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY; v. 124, n. 2, p. 175-182, AUG 2017.
Web of Science Citations: 2
Abstract

Objective. Recent studies suggested that head and neck radiotherapy increases active forms of matrix metalloproteinases (MMPs) in the dentin-enamel junction (DEJ), leading to enamel delamination and radiation-related caries. This study aimed to assess the expression and activity of the gelatinases MMP-2 and MMP-9 in the DEJ and dentin-pulp complex tissues of teeth irradiated in vivo. Study Design. Thirty-six teeth were studied, including 19 irradiated and 17 non-irradiated controls. In situ zymography was used to investigate the gelatinolytic activity in the micromorphologic components of enamel, DEJ, dentin-pulp complex, and caries. Immunohistochemical analysis was conducted on the demineralized samples to assess MMP-2 and MMP-9 expression levels in the DEJ, dentin-pulp complex components, and caries. Results. No statistically significant differences were detected between groups in gelatinolytic activity or in MMP-2 expression levels (P > .05). Odontoblast MMP-9 expression was reduced in the irradiated group (P = .02). Conclusions. The study rejected the hypothesis that MMP-2 and MMP-9 would be overexpressed or more activated in the DEJ and dentin-pulp complex of irradiated teeth. Direct effects of radiation should not be regarded as an independent factor for explaining radiation-related caries onset and progression. (AU)

FAPESP's process: 12/06138-1 - A prospective study of radiation-induced trismus in patients with oral squamous cell carcinoma
Grantee:Ana Carolina Prado Ribeiro e Silva
Support Opportunities: Regular Research Grants
FAPESP's process: 13/18402-8 - Evaluation of the direct effects of radiation on the enamel, dentin and dental pulp of head and neck cancer patients
Grantee:Alan Roger dos Santos Silva
Support Opportunities: Regular Research Grants