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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

A DiCre recombinase-based system for inducible expression in Leishmania major

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Santos, Renato E. R. S. [1] ; Silva, Gabriel L. A. [1] ; Santos, Elaine V. [1] ; Duncan, Samuel M. [2] ; Mottram, Jeremy C. [2, 3] ; Damasceno, Jeziel D. [1] ; Tosi, Luiz R. O. [1]
Total Authors: 7
[1] Univ Sao Paulo, Dept Cell & Mol Biol, Ribeirao Preto Med Sch, Ribeirao Preto, SP - Brazil
[2] Univ Glasgow, Inst Infect Immun & Inflammat, Wellcome Ctr Mol Parasitol, Glasgow, Lanark - Scotland
[3] Univ York, Dept Biol, Ctr Immunol & Infect, York, N Yorkshire - England
Total Affiliations: 3
Document type: Journal article
Source: Molecular and Biochemical Parasitology; v. 216, p. 45-48, SEP 2017.
Web of Science Citations: 4

Here we present the establishment of an inducible system based on the dimerizable Cre recombinase (DiCre) for controlled gene expression in the protozoan parasite Leishmania. Rapamycin-induced DiCre activation promoted efficient flipping and expression of gene products in a time and dose-dependent manner. The DiCre flipping activity induced the expression of target genes from both integrated and episomal contexts broadening the applicability of the system. We validated the system by inducing the expression of both full length and truncated forms of the checkpoint protein Rad9, which revealed that the highly divergent C-terminal domain of Rad9 is necessary for proper subcellular localization. Thus, by establishing the DiCre-based inducible system we have created and validated a robust new tool for assessing gene function in Leishmania. (AU)

FAPESP's process: 09/54014-7 - Acquisition of a biophotonic imaging system and a multiphoton microscopy system for in vivo imaging
Grantee:Enilza Maria Espreafico
Support type: Multi-user Equipment Program
FAPESP's process: 14/00751-9 - Identification of LmRad9, LmHus1 and LmRad1-interacting proteins and generation of a LmHus1 conditional knockout cell line
Grantee:Jeziel Dener Damasceno
Support type: Scholarships abroad - Research Internship - Post-doctor
FAPESP's process: 13/00570-1 - Characterization of the putative 9-1-1 complex of Leishmania major
Grantee:Jeziel Dener Damasceno
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 14/06824-8 - Characterization of the proteins LmRad9 and LmRad1 in the DNA damage response of Leishmania major
Grantee:Luiz Ricardo Orsini Tosi
Support type: Regular Research Grants
FAPESP's process: 13/26806-1 - Characterization of LmRad1 of Leishmania major and study of its role in the DNA damage response
Grantee:Elaine Vieira Santos
Support type: Scholarships in Brazil - Doctorate