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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Nitric oxide-releasing nanoparticles: synthesis, characterization, and cytotoxicity to tumorigenic cells

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Pelegrino, Milena T. [1, 2] ; Silva, Letecia C. [2] ; Watashi, Carolina M. [2] ; Haddad, Paula S. [1] ; Rodrigues, Tiago [2] ; Seabra, Amedea B. [2]
Total Authors: 6
[1] Univ Fed Sao Paulo, Exact & Earth Sci Dept, Rua Sao Nicolau 210, BR-09913030 Diadema, SP - Brazil
[2] Univ Fed ABC, UFABC, Ctr Nat & Human Sci, Ave Estados 5001, CEP Bairro Bangu Bloco A, Torre 3, BR-09210580 Santo Andre, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Web of Science Citations: 12

Nitric oxide (NO) is involved in several biological processes, including toxicity against tumor cells. The aim of this study was to synthesize, characterize, and evaluate the cytotoxicity of NO-releasing chitosan nanoparticles. A thiol-containing molecule, mercaptosuccinic acid (MSA), was encapsulated (encapsulation efficiency of 99%) in chitosan/sodium tripolyphosphate nanoparticles (CS NPs). The obtained nanoparticles showed an average hydrodynamic size of 108.40 +/- 0.96 nm and polydispersity index of 0.26 +/- 0.01. MSA-CS NPs were nitrosated leading to S-nitroso-MSA-CS NPs, which act as NO donor. The cytotoxicity of CS NPs, MSA-CS NPs, and S-nitrosoMSA- CS NPs were evaluated in several tumor cells, including human hepatocellular carcinoma (HepG2), mouse melanoma (B16F10), and human chronic myeloid leukemia (K562) cell lines and Lucena-1, a vincristine-resistant K562 cell line. Both CS NPs and MSA-CS NPs did not cause toxic effects in these cells, whereas S-nitroso-MSA-CS NPs caused potent cytotoxic effects in all the tested tumor cell lines. The halfmaximal inhibitory concentration values of S-nitroso-MSA-CS NPs were 19.7, 10.5, 22.8, and 27.8 mu g.mL(-1) for HepG2, B16F10, K562, and Lucena-1 cells, respectively. In contrast, S-nitroso-MSA-CS NPs exhibited lower cytotoxic to non-tumorigenic melanocytes (Melan-A) when compared with melanoma B16F10. Therefore, the results highlight the potential use of NO-releasing CS NPs in antitumor chemotherapy. (AU)

FAPESP's process: 14/13913-7 - Preparation, characterization and biological evaluations of mono and bimetallic nanoparticles systems
Grantee:Paula Silvia Haddad Ferreira
Support type: Regular Research Grants
FAPESP's process: 15/00393-8 - Nitric oxide releasing polymeric nanoparticles for topical applications
Grantee:Milena Trevisan Pelegrino
Support type: Scholarships in Brazil - Master
FAPESP's process: 16/10347-6 - S-nitrosothiol containing nanoparticles: Synthesis, characterization, cytotoxicity and applications
Grantee:Amedea Barozzi Seabra
Support type: Regular Research Grants