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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Aptamer-based Biosensor Developed to Monitor MUC1 Released by Prostate Cancer Cells

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Author(s):
Karpik, Agnieszka E. [1] ; Crulhas, Bruno P. [2] ; Rodrigues, Carolina B. [2] ; Castro, Gustavo R. [2] ; Pedrosa, Valber A. [2]
Total Authors: 5
Affiliation:
[1] Inst Mat Sci & Engn, Fac Mech Engn, Lodz - Poland
[2] UNESP, Dept Chem & Biochem, Inst Biosci, Botucatu, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Electroanalysis; v. 29, n. 10, p. 2246-2253, OCT 2017.
Web of Science Citations: 8
Abstract

Electrochemical aptasensors can detect cancer biomarkers such as mucin 1 (MUC1) to provide point-of-care diagnosis that is low-cost, specific and sensitive. Herein, a DNA hairpin containing MUC1 aptamer was thiolated, conjugated with methylene blue (MB) redox tag, and immobilized on a gold electrode by self-assembly. The fabrication process was characterized by scanning electron microscopy, X-ray spectroscopy analysis and electrochemistry techniques. The results evidenced a stable and sensitivity sensor presenting wide linear detection range (0.65-110 ng/mL). Therefore, it was able to precisely detect MUC1 production patterns in normal (RWPE-1) and prostate cancer cells (LNCaP and PC3). The biosensor has ability to detect MUC1 in complex samples being an efficient and useful platform for cancer diagnosing in early stages and for physiological applications such as cancer treatment monitoring. (AU)

FAPESP's process: 16/15919-8 - Monitoring of prostate cancer biomarkers using a biosensor
Grantee:Valber de Albuquerque Pedrosa
Support type: Regular Research Grants