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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Conjugation with L, L-diphenylalanine Self-Assemblies Enhances In Vitro Antitumor Activity of Phthalocyanine Photosensitizer

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Author(s):
Souza, Marcia I. [1] ; Prieto, Tatiana [1] ; Rodrigues, Tiago [1] ; Ferreira, Fabio F. [1] ; Nascimento, Francisco B. [1] ; Ribeiro, Anderson O. [1] ; Silva, Emerson R. [2] ; Giuntini, Francesca [3] ; Alves, Wendel A. [1]
Total Authors: 9
Affiliation:
[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, BR-09210580 Santo Andre, SP - Brazil
[2] Univ Fed Sao Paulo, Dept Biofis, BR-04023062 Sao Paulo - Brazil
[3] Liverpool John Moores Univ, Sch Pharm & Biomol Sci, Byrom St, Liverpool L3 3AF, Merseyside - England
Total Affiliations: 3
Document type: Journal article
Source: SCIENTIFIC REPORTS; v. 7, OCT 13 2017.
Web of Science Citations: 3
Abstract

We present the synthesis and characterization of new peptide conjugates obtained by hierarchical co-assembly of L, L-diphenylalanine (FF) and zinc phthalocyanine complexes (ZnPc) in water. Self-assembly capabilities under defined conditions were investigated by scanning electron microscopy, and photophysical properties were evaluated using UV-Vis and fluorescence spectroscopy. AFM observations demonstrated that these ZnPcs form different highly ordered arrays on the crystalline faces of the FF microplates and that surface roughness significantly changes with the presence of differently substituted phthalocyanine units. XRD assays showed that the overall molecular packing of the conjugates is organized according to a hexagonal symmetry, with ZnPcs hosted in the interstices of the peptide phase. In vitro photodynamic studies were conducted on human breast cancer MCF-7 cells to investigate both cellular uptake and cytotoxicity. It was shown that FF self-assemblies are not toxicity and enhance accumulation of ZnPc in MCF-7 cells, improving apoptotic cell death upon irradiation. Our findings demonstrate enhancement of ZnPc antitumor efficiency by FF conjugates and a proof-of-concept for new photosensitizer carriers based on peptide conjugates. (AU)

FAPESP's process: 15/24018-1 - Polymer-Peptide Conjugates for hydrogel formulation and its usage in detection
Grantee:Wendel Andrade Alves
Support Opportunities: Regular Research Grants
FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
Grantee:Marco Aurelio Zezzi Arruda
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 14/50972-1 - Francesca Giuntini | Liverpool John Moores University - Inglaterra
Grantee:Wendel Andrade Alves
Support Opportunities: Research Grants - Visiting Researcher Grant - International
FAPESP's process: 16/24409-3 - Cell-Penetrating Peptides for Transport of Plasmid DNA and microRNA: from Nanoscopic Structure to Gene Delivery.
Grantee:Emerson Rodrigo da Silva
Support Opportunities: Regular Research Grants