| Full text | |
| Author(s): |
Elizete Aparecida LOMAZI
[1]
;
Nelson Ary BRANDALISE
[2]
;
Maria de Fátima Pimenta Correa SERVIDONI
[3]
;
Silvia Regina CARDOSO
[4]
;
Luciana Rodrigues MEIRELLES
[5]
Total Authors: 5
|
| Affiliation: | [1] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Pediatria - Brasil
[2] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Cirurgia - Brasil
[3] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Pediatria - Brasil
[4] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Pediatria - Brasil
[5] Universidade Estadual de Campinas. Faculdade de Ciências Médicas. Departamento de Patologia - Brasil
Total Affiliations: 5
|
| Document type: | Journal article |
| Source: | Arq. Gastroenterol.; v. 54, n. 3, p. 192-196, 2017-05-08. |
| Abstract | |
ABSTRACT BACKGROUND: Mast cells exert a substantial role in gastrointestinal allergic diseases. Therefore, it is reasonable to presume that mast cell may aid diagnosis in eosinophilic gastroenteropathy. OBJECTIVE: To evaluate whether mast cell count in the esophageal epithelium can discriminate eosinophilic esophagitis, proton-pump inhibitor (PPI)-responsive eosinophilic esophagitis and gastroesophageal reflux esophagitis. METHODS: Retrospectively we reviewed the files of 53 consecutive patients (age: 7.8 years; range: 8-14 years) with definitive diagnose established during clinical follow up in a universitary outpatient clinic as follow: eosinophilic esophagitis (N=23), PPI-responsive eosinophilic esophagitis (N=15) and gastroesophageal reflux esophagitis (N=15). Eosinophil count in the esophageal epithelium in slides stained with H-E was reviewed and immunohistochemistry for mast cell tryptase was performed. RESULTS: Count of eosinophils/high-power field (HPF) higher than 15 were found in 14 out of 15 reflux esophagitis patients. The mean count of eosinophils/HPF was similar in eosinophilic esophagitis patients and in those with PPI-responsive eosinophilic esophagitis (42 and 39 eosinophils/HPF, respectively, P=0.47). Values of mast cell tryptase (+) were higher in eosinophilic esophagitis [median: 25 mast cells/HPF; range (17-43) ] and in PPI-responsive eosinophilic esophagitis patients [25 (16-32) ], compared to reflux esophagitis [4 (2-14) ], P<0.001. There was no difference between the mean count of mast cells/HPF in the esophageal epithelium of eosinophilic esophagitis patients and PPI-responsive eosinophilic esophagitis patients, respectively, 26 and 24 mast cells/HPF, P=0.391. CONCLUSION: Tryptase staining of mast cells differentiates eosinophilic esophagitis from reflux esophagitis. (AU) | |