Effects of photobiomodulation therapy and topical non-steroidal anti-inflammatory drug on skeletal muscle injury induced by contusion in rats-part 1: morphological and functional aspects

Author(s):Show less -

Tomazoni, Shaiane Silva ^{[1, 2]} ; Frigo, Lucio ^[3] ; dos Reis Ferreira, Tereza Cristina ^{[4, 5]} ; Casalechi, Heliodora Leo ^[5] ; Teixeira, Simone ^[6] ; de Almeida, Patricia ^[7] ; Muscara, Marcelo Nicolas ^[6] ; Marcos, Rodrigo Labat ^[7] ; Serra, Andrey Jorge ^[7] ; de Carvalho, Paulo de Tarso Camillo ^{[4, 7]} ; Pinto Leal-Junior, Ernesto Cesar ^{[4, 5]}

Affiliation

^[1] Univ Cidade Sao Paulo UNICID, Masters Program Phys Therapy, Rua Cesario Galeno 448-475, BR-05508900 Sao Paulo, SP - Brazil

^[2] Univ Cidade Sao Paulo UNICID, Doctoral Program Phys Therapy, Rua Cesario Galeno 448-475, BR-05508900 Sao Paulo, SP - Brazil

^[3] Cruzeiro Sul Univ UNICSUL, Biol Sci & Hlth Ctr, Sao Paulo, SP - Brazil

^[4] Nove de Julho Univ UNINOVE, Postgrad Program Rehabil Sci, Sao Paulo, SP - Brazil

^[5] Nove de Julho Univ UNINOVE, Lab Phototherapy Sports & Exercise, Sao Paulo, SP - Brazil

^[6] Univ Sao Paulo, Inst Biomed Sci, Dept Pharmacol, Sao Paulo, SP - Brazil

^[7] Nove de Julho Univ UNINOVE, Postgrad Program Biophoton Appl Hlth Sci, Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: Lasers in Medical Science; v. 32, n. 9, p. 2111-2120, DEC 2017.
• Web of Science Citations: 8

Abstract

Musculoskeletal injuries are very frequent and are responsible for causing pain and impairment of muscle function, as well as significant functional limitations. In the acute phase, the most prescribed treatment is with non-steroidal anti-inflammatory drugs (NSAIDs), despite their questionable effectiveness. However, the use of photobiomodulation therapy (PBMT) in musculoskeletal disorders has been increasing in the last few years, and this therapy appears to be an interesting alternative to the traditional drugs. The objective of the present study was to evaluate and compare the effects of PBMT, with different application doses, and topical NSAIDs, under morphological and functional parameters, during an acute inflammatory process triggered by a controlled model of musculoskeletal injury induced via contusion in rats. Muscle injury was induced by means of a single trauma to the animals' anterior tibialis muscle. After 1 h, the rats were treated with PBMT (830 nm; continuous mode, with a power output of 100 mW; 3.57 W/cm(2); 1 J-35.7 J/cm(2), 3 J-107.1 J/cm(2), and 9 J-321.4 J/cm(2); 10, 30, and 90 s) or diclofenac sodium for topical use (1 g). Morphological analysis (histology) and functional analysis (muscle work) were performed, 6, 12, and 24 h after induction of the injury. PBMT, with all doses tested, improved morphological changes caused by trauma; however, the 9 J (321.4 J/cm(2)) dose was the most effective in organizing muscle fibers and cell nuclei. On the other hand, the use of diclofenac sodium produced only a slight improvement in morphological changes. Moreover, we observed a statistically significant increase of muscle work in the PBMT 3 J (107.1 J/cm(2)) group in relation to the injury group and the diclofenac group (p < 0.05). The results of the present study indicate that PBMT, with a dose of 3 J (107.1 J/cm(2)), is more effective than the other doses of PBMT tested and NSAIDs for topical use as a means to improve morphological and functional alterations due to muscle injury from contusion. (AU)

• FAPESP's process: 12/06832-5 - Low-level laser therapy and topical sodium diclofenac in contusion-induced skeletal muscle injury in rats
• Grantee: Lúcio Frigo
• Support Opportunities: Regular Research Grants