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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Study of Microbial Interaction Formed by "Candida krusei" and "Candida glabrata": "In Vitro" and "In Vivo" Studies

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Author(s):
Rodnei Dennis Rossoni [1] ; Patrícia Pimentel de Barros [2] ; Fernanda Freire [3] ; Jéssica Diane dos Santos [4] ; Antonio Olavo Cardoso Jorge [5] ; Juliana Campos Junqueira [6]
Total Authors: 6
Affiliation:
[1] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
[2] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
[3] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
[4] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
[5] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
[6] Universidade Estadual Paulista. Department of Biosciences and Oral Diagnosis. Institute of Science and Technology - Brasil
Total Affiliations: 6
Document type: Journal article
Source: Brazilian Dental Journal; v. 28, n. 6, p. 669-674, 2017-12-00.
Abstract

Abstract Recently, the non-albicans Candida species have become recognized as an important source of infection and oral colonization by association of different species in a large number of immunosuppressed patients. The objective of this study was to evaluate the interactions between C. krusei and C. glabrata in biofilms formed in vitro and their ability to colonize the oral cavity of mouse model. Monospecies and mixed biofilms were developed of each strain, on 96-well microtiter plates for 48 h. These biofilms were analyzed by counting colony-forming units (CFU/mL) and by determining cell viability, using the XTT hydroxide colorimetric assay. For the in vivo study, twenty-four mice received topical applications of monospecie or mixed suspensions of each strain. After 48 h, yeasts were recovered from the mice and quantified by CFU/mL count. In the biofilm assays, the results for the CFU/mL count and the XTT assay showed that the two species studied were capable of forming high levels of in vitro monospecie biofilm. In mixed biofilm, the CFU of C. krusei increased (p=0.0001) and C. glabrata decreased (p=0.0001). The metabolic activity observed in XTT assay of mixed biofilm was significantly reduced compared with a single C. glabrata biofilm (p=0.0001). Agreeing with CFU in vitro count, C. glabrata CFU/mL values recovered from oral cavity of mice were statistically higher in the group with single infection (p=0.0001) than the group with mixed infection. We concluded that C. krusei inhibits C. glabrata and takes advantage to colonize the oral cavity and to form biofilms. (AU)

FAPESP's process: 13/25181-8 - Isolation and characterization of Lactobacillus spp. from oral cavity and their probiotic action in Candida albicans: biofilm formation, infection in invertebrate models and EFG1, HWP1 and ALS1 gene expression
Grantee:Rodnei Dennis Rossoni
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 12/02184-9 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
Grantee:Juliana Campos Junqueira
Support Opportunities: Regular Research Grants