CD105 is regulated by hsa-miR-1287 and its expression is inversely correlated with osteopotential in SHED

Author(s)

Andre Ishiy, Felipe Augusto ^[1] ; Fanganiello, Roberto Dalto ^[1] ; Kobayashi, Gerson Shigeru ^[1] ; Kague, Erika ^[1] ; Kuriki, Patricia Semedo ^[1] ; Passos-Bueno, Maria Rita ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Genet & Evolucao, Sao Paulo - Brazil

• Document type: Journal article
• Source: BONE; v. 106, p. 112-120, JAN 2018.
• Web of Science Citations: 5

Abstract

A more accurate understanding of the molecular mechanisms and signaling pathways underpinning human mesenchymal stem cell (MSC) plasticity and differentiation properties is pivotal for accomplishing solid and diligent translation of MSC-based experimental therapeutics and clinical trials to broad clinical practice. In addition, this knowledge enables selection of MSC subpopulations with increased differentiation potential and/or use of exogenous factors to boost this potential. Here, we report that CD105 (ENG) is a predictive biomarker of osteogenic potential in two types of MSCs: stem cells from human exfoliated deciduous teeth (SHED) and human adipose-derived stem cells (hASC). We also validate that CD105 can be used to select and enrich for subpopulations of SHED and hASC with higher in vitro osteogenic potential. In addition, we show that hsa-mir-1287 regulates CD105 expression, and propose that fine-tuning hsa-mir-1287 levels could be used to control osteopotential in SHED. These findings provide better discernment of the molecnlar bases behind MSC osteogenic plasticity and open up need perspectives to leverage osteogenic potential in MSCs by modulation of a specific miRNA. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• FAPESP's process: 13/08028-1 - CEGH-CEL - Human Genome and Stem Cell Research Center
• Grantee: Mayana Zatz
• Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC