Paper-Based Microfluidics Immunoassay for Detection of Canine Distemper Virus

Author(s)

Thiago Mazzu-Nascimento ^{[1, 2]} ; Fabiana Cristina Donofrio ^[3] ; Beatriz Cutilak Bianchi ^[4] ; Regiane de Fátima Travensolo ^[4] ; João Batista Souza Junior ^[5] ; Daniel Angeli Moraes ^[1] ; Giorgio Gianini Morbioli ^{[1, 2, 7]} ; Thiago Pinotti Segato ^[4] ; Laudemir Carlos Varanda ^[1] ; Emanuel Carrilho ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Quim Sao Carlos, BR-13566590 Sao Carlos, SP - Brazil

^[2] Inst Nacl Ciencia & Tecnol Bioanalit, Campinas, SP - Brazil

^[3] Univ Fed Mato Grosso, Inst Ciencias Saude, BR-78557267 Sinop, MT - Brazil

^[4] Partecurae Pesquisa & Desenvolvimento, BR-13560460 Sao Carlos, SP - Brazil

^[5] Partecurae Pesquisa e Desenvolvimento - Brasil

^[6] Universidade de São Paulo. Instituto de Química de São Carlos - Brasil

^[7] Georgia Inst Technol, Sch Chem & Biochem, Atlanta, GA 30332 - USA

• Document type: Journal article
• Source: Brazilian Archives of Biology and Technology; v. 60, 2018-01-08.

Abstract

ABSTRACT Paper-based devices present low-cost and are versatile, making them very attractive for clinical analysis. To manufacture those devices wax patterns are printed on paper surface and upon heating the wax permeates through the entire thickness of the paper, creating hydrophobic barriers that delimit test areas. Antibodies produced in rabbits against canine distemper virus (CDV) were physically adsorbed on the surface of gold nanoparticles (AuNPs) and incubated with CDV viral antigens, forming the immunocomplex. Anti-CDV antibodies were immobilized into the microchannels by physical adsorption, forming the test region. The test solution containing conjugated AuNPs was applied at the bottom of the microchannel and it was eluted with a phosphate buffer solution 0.01 M pH 7.4. When the solution containing the AuNPs reached the test zone the recognition of antigens contained on the immunocomplex occurred with the consequent development of a red line, which represents a positive outcome for the test. This method demonstrated the success of physical immobilization of antibodies on AuNPs and the physical immobilization of antibodies on cellulose’s surface. This colorimetric assay brings simplicity and versatility to clinical analyses, presenting potential for CDV diagnosis. (AU)

• FAPESP's process: 12/02093-3 - Synthesis of Co@Au (superparamagnetics) and CdSe@Zn (quantum dots) core@shell nanostructures for applications as antineoplasia agents
• Grantee: João Batista Souza Junior
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 11/13997-8 - Paper-based platforms for noninvasive detection of tumor biomarkers and cultive neoplastic cells
• Grantee: Thiago Mazzu Do Nascimento
• Support Opportunities: Scholarships in Brazil - Doctorate (Direct)