Development of microsatellite loci for Cryptocarya... - BV FAPESP
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(Reference retrieved automatically from SciELO through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues

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Author(s):
Thabata Carvalho [1] ; Giuliana Garcia [2] ; Carolina Silva Carvalho [3] ; Clarisse Palma-Silva [4] ; Laurence Culot [5]
Total Authors: 5
Affiliation:
[1] Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Departamento de Zoologia - Brasil
[2] Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Departamento de Zoologia - Brasil
[3] Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Departamento de Ecologia - Brasil
[4] Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Departamento de Ecologia - Brasil
[5] Universidade Estadual Paulista "Júlio de Mesquita Filho". Instituto de Biociências. Departamento de Zoologia - Brasil
Total Affiliations: 5
Document type: Journal article
Source: Hoehnea; v. 44, n. 4, p. 580-587, 2017-12-00.
Abstract

ABSTRACT Nine polymorphic microsatellite loci were isolated and characterized for Cryptocarya mandioccana Meisner, a tree from the Atlantic Rainforest with seeds dispersed by large animals. The loci were characterized using 48 individuals from two populations and their genotyping success tested in four tissues: leaves from adults and seedlings, and two diaspore maternal tissues. Maternity analyses were also performed on diaspores and leaves from nine adult trees. The number of alleles per locus ranged from nine to 15 and the observed and expected heterozygosities ranged from 0.214 to 0.864 and 0.745 to 0.892, respectively. The loci genotyping success did not significantly differ between tissues and varied from 56 to 96%. The microsatellites showed enough polymorphism to assign the nine adult trees to their diaspores. The successful genotyping in all tissues and identification of mother trees show that the microsatellites are suitable for studies such as spatial genetic structure and maternity analyses. (AU)

FAPESP's process: 14/01029-5 - Ecological and genetics effects of seed size variation in a defaunated landscape
Grantee:Carolina da Silva Carvalho
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 09/52725-3 - Speciation, reproductive isolation, and population genetics in species of Bromeliaceae family: implications for taxonomy, evolution and conservation
Grantee:Clarisse Palma da Silva
Support Opportunities: BIOTA-FAPESP Program - Young Investigators Grants
FAPESP's process: 14/20621-2 - Effects of defaunation on the spatial genetic structure of a species of tree in the Atlantic forest
Grantee:Thabata Rodrigues de Carvalho
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 14/14739-0 - The effect of fragmentation on primate ecological functions
Grantee:Laurence Marianne Vincianne Culot
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 14/20622-9 - Evaluation of seed dispersal distance of a Mata Atlântica plant species: comparison between primates and birds
Grantee:Giuliana Garcia
Support Opportunities: Scholarships in Brazil - Scientific Initiation
FAPESP's process: 10/16075-1 - Cascading effects of defaunation on seed dispersal process: primate - plant interactions in the Atlantic rainforest
Grantee:Laurence Marianne Vincianne Culot
Support Opportunities: Scholarships in Brazil - Post-Doctoral