Employing Calcination as a Facile Strategy to Reduce the Cytotoxicity in CoFe2O4 and NiFe2O4 Nanoparticles

Author(s)

Lima, Debora R. ^{[1, 2]} ; Jiang, Ning ^[3] ; Liu, Xin ^[4] ; Wang, Jiale ^[5] ; Vulcani, Valcinir A. S. ^{[1, 2]} ; Martins, Alessandro ^{[1, 2]} ; Machado, Douglas S. ^{[1, 2]} ; Landers, Richard ^[6] ; Camargo, Pedro H. C. ^[7] ; Pancotti, Alexandre ^{[1, 2]}

Affiliation

^[1] Univ Fed Goias, Unidade Acad Especial Ciencias Exatas, Reg Jatai, Rod Br 364, km 168, BR-76600000 Jatai, Go - Brazil

^[2] Univ Fed Goias, Unidade Acad Especial Ciencias Saude, Rod Br 364, km 168, BR-76600000 Jatai, Go - Brazil

^[3] Shanghai Jiao Tong Univ, Sch Med, Shanghai Key Lab Stomatol, Peoples Hosp 9, Coll Stomatol, Dept Oral & Craniomaxillofacial Sci, Shanghai 200011 - Peoples R China

^[4] Shandong Jiaotong Univ, Sch Med, Shanghai Key Lab Stomatol, Peoples Hosp 9, Shanghai Biomat Res & Testing Ctr, 427 Ju Men Rd, Shanghai 200023 - Peoples R China

^[5] Donghua Univ, Coll Sci, Shanghai 201620 - Peoples R China

^[6] Univ Estadual Campinas, Inst Fis Gleb Wataghin, BR-13083859 Campinas, SP - Brazil

^[7] Univ Sao Paulo, Inst Quim, Dept Quim Fundamental, Av Lineu Prestes 748, BR-05505000 Sao Paulo, SP - Brazil

• Document type: Journal article
• Source: ACS APPLIED MATERIALS & INTERFACES; v. 9, n. 45, p. 39830-39838, NOV 15 2017.
• Web of Science Citations: 5

Abstract

CoFe2O4 and NiFe2O4 nanoparticles (NPs) represent promising candidates for biomedical applications. However, in these systems, the knowledge over how various physical and chemical parameters influence their cytotoxicity remains limited. In this article, we investigated the effect of different calcination temperatures over cytotoxicity of CoFe2O4 and NiFe2O4 NPs, which were synthesized by a sol-gel proteic approach, toward L929 mouse fibroblastic cells. More specifically, we evaluated and compared CoFe2O4 and NiFe2O4 NPs presenting low crystallinity (that were calcined at 400 and 250 degrees C, respectively) with their highly crystalline counterparts (that were calcined at 800 degrees C). We found that the increase in the calcination temperature led to the reduction in the concentration of surface defect sites and/or more Co or Ni atoms located at preferential crystalline sites in both cases. A reduction in the cytotoxicity toward mouse fibroblast L929 cells was observed after calcination at 800 degrees C. Combining with inductively coupled plasma mass spectrometry data, our results indicate that the calcination temperature can be employed as a facile strategy to reduce the cytotoxicity of CoFe2O4 and NiFe2O4, in which higher temperatures contributed to the decrease in the dissolution of Co2+ or Ni2+ from the NPs. We believe these results may shed new insights into the various parameters that influence cytotoxicity in ferrite NPs, which may pave the way for their widespread applications in biomedicine. (AU)

• FAPESP's process: 15/21366-9 - Hybrid materials containing metal nanoparticles for catalytic applications
• Grantee: Pedro Henrique Cury Camargo
• Support type: Regular Research Grants
• FAPESP's process: 15/26308-7 - Optimization of the physicochemical properties of nano -structured materials for applications in molecular recognition, catalysis and energy conversion/storage
• Grantee: Roberto Manuel Torresi
• Support type: Research Projects - Thematic Grants