Involvement of Opioid System, TRPM8, and ASIC Receptors in Antinociceptive Effect of Arrabidaea brachypoda (DC) Bureau

Author(s):Show less -

Rodrigues, Vinicius Peixoto ^[1] ; da Rocha, Claudia Quintino ^[2] ; Perico, Larissa Lucena ^[1] ; dos Santos, Raquel de Cassia ^[3] ; Ohara, Rie ^[1] ; Nishijima, Catarine Massucato ^[1] ; Queiroz, Emerson Ferreira ^[4] ; Wolfender, Jean-Luc ^[4] ; Machado da Rocha, Lucia Regina ^[1] ; Soares Santos, Adair Roberto ^[5] ; Vilegas, Wagner ^[6] ; Hiruma-Lima, Clelia Akiko ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Biosci Inst, Dept Physiol, BR-18618970 Botucatu, SP - Brazil

^[2] Univ Fed Maranhao, Dept Chem, Ave Portugueses 1966, BR-65080805 Sao Luis, MA - Brazil

^[3] Clin Pharmacol & Gastroenterol Unit USF, BR-12916900 Braganca Paulista, SP - Brazil

^[4] Univ Lausanne, Univ Geneva, Sch Pharmaceut Sci, EPGL, CMU Rue Michel Servet 1, CH-1211 Geneva 4 - Switzerland

^[5] Univ Fed Santa Catarina, Dept Physiol Sci, Ctr Biol Sci, Lab Neurobiol Pain & Inflammat, BR-88040900 Florianopolis, SC - Brazil

^[6] Sao Paulo State Univ UNESP, Biosci Inst, BR-11330900 Sao Vicente, SP - Brazil

• Document type: Journal article
• Source: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES; v. 18, n. 11 NOV 2017.
• Web of Science Citations: 1

Abstract

Arrabidaea brachypoda (DC) Bureau is a medicinal plant found in Brazil. Known as cipo-una, it is popularly used as a natural therapeutic agent against pain and inflammation. This study evaluated the chemical composition and antinociceptive activity of the dichloromethane fraction from the roots of A. brachypoda (DEAB) and its mechanism of action. The chemical composition was characterized by high-performance liquid chromatography, and this fraction is composed only of dimeric flavonoids. The antinociceptive effect was evaluated in formalin and hot plate tests after oral administration (10-100 mg/kg) in male Swiss mice. We also investigated the involvement of TRPV1 (transient receptor potential vanilloid 1), TRPA1 (transient receptor potential ankyrin 1), TRPM8 (transient receptor potential melastatin 8), and ASIC (acid-sensing ion channel), as well as the opioidergic, glutamatergic, and supraspinal pathways. Moreover, the nociceptive response was reduced (30 mg/kg) in the early and late phase of the formalin test. DEAB activity appears to involve the opioid system, TRPM8, and ASIC receptors, clearly showing that the DEAB alleviates acute pain in mice and suggesting the involvement of the TRPM8 and ASIC receptors and the opioid system in acute pain relief. (AU)

• FAPESP's process: 09/52237-9 - Standardized herbal medicines for the treatment of chronic diseases
• Grantee: Wagner Vilegas
• Support Opportunities: BIOTA-FAPESP Program - Thematic Grants