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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Microbial analysis of root canal and periradicular lesion associated to teeth with endodontic failure

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Author(s):
Pereira, R. S. [1] ; Rodrigues, V. A. A. [2] ; Furtado, W. T. [1] ; Gueiros, S. [2] ; Pereira, G. S. [1] ; Avila-Campos, M. J. [2]
Total Authors: 6
Affiliation:
[1] Univ Fed Espirito Santo, Dept Dent Clin, Discipline Endodont, Vitoria, ES - Brazil
[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Anaerobe Lab, Sao Paulo, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Anaerobe; v. 48, p. 12-18, DEC 2017.
Web of Science Citations: 5
Abstract

The quantification of ten microorganisms at the root ends and in the surrounding periradicular lesions was performed. Thirty 3 mm samples root ends and 30 samples of the surrounding chronic periapical infection were collected during apical microsurgery. Samples were triturated, and the bacterial DNA was obtained. The bacterial quantification was performed by using the SYBR Green system. At least one microorganism was detected in all patients. In both the root end and periapical samples, Fusobacterium nucleatum (71.6%), Dialister pneumosintes (58.3%) and Tannerella forsythia (48.3%) were the most prevalent species. Dialister pneumosintes showed statistically significant values in the root end, and F. nucleatum was also significant in the apical periodontitis samples. A statistically significant association between T. forsythia and Porphyromonas gingivalis in the root ends was observed. Bacterial associations from 2 to 7 species were observed in most samples. Extra-radicular and/or intra-radicular infections were present in all teeth with failed endodontic treatment, and showed polymicrobial infection in most cases, with a predominance of E nucleatum, D. pneumosintes and T forsythia. When present, Enterococcus faecalis was never found to be the most prevalent species. The presence of a microbial diversity in post-treatment apical periodontitis confirms the polymicrobial and synergistic characteristic of this process. Our results show that the bacterial array associated with the 3 mm root ends and periradicular lesions in post-treatment apical periodontitis are complex and with a high inter-individual variability. These results might be useful to delineate treatment strategies for microbial elimination in apical periodontitis. Further studies are necessary to elucidate the role of these microorganisms in endodontic treatment failures. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 13/13652-6 - Phenotypic and genotypic profiles of Aggregatibacter actinomycetemcomitans e Fusobacterium nucleatum from patients with different clinical status: qualitative and quantitative determinations
Grantee:Mario Júlio Ávila-Campos
Support Opportunities: Regular Research Grants