Genome sequence analysis of a hypermucoviscous/hypervirulent and MDR CTX-M-15/K19/ST29 Klebsiella pneumoniae isolated from human infection

Author(s)

Moura, Quezia ^[1] ; Esposito, Fernanda ^[2] ; Fernandes, Miriam R. ^[2] ; Espinoza-Munoz, Maria ^[2] ; Souza, Tiago A. ^[3] ; Santos, Silvia R. ^[4] ; Cerdeira, Louise ^[2] ; Cassettari, Valeria ^[5] ; Lincopan, Nilton ^{[1, 2]}

Affiliation

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Sch Pharm, Dept Clin Anal, BR-05508000 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Genome Invest & Anal Lab GENIAL, BR-05508000 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Univ Hosp, Lab Clin Anal, BR-05508000 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Univ Hosp, Hosp Infect Control Comm, BR-05508000 Sao Paulo - Brazil

• Document type: Journal article
• Source: PATHOGENS AND DISEASE; v. 75, n. 9 DEC 2017.
• Web of Science Citations: 3

Abstract

The emergence of hypervirulent Klebsiella pneumoniae (hvKP) with multidrug resistance (MDR) profile is a worrisome public health issue. We report the first draft genome sequence of a hypermucoviscous (positive string test) and MDR K. pneumoniae serotype K19, belonging to ST29, isolated from human infection. This strain harboured multiple antimicrobial resistance genes, including blaCTX-M-15, besides yersiniabactin and type 3 fimbriae virulence genes. In vivo experiments carried out with the Galleria mellonella infection model revealed that K. pneumoniae K19/ST29 killed 100% of the larvae at 24 h post-infection, in a similar way to the known hypermucoviscous hvKP K1/ST23 lineage. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants