| Full text | |
| Author(s): |
Preite, Nycolas Willian
[1]
;
Feriotti, Claudia
[1]
;
de Lima, Dhemerson Souza
[1]
;
da Silva, Bruno Borges
[1]
;
Condino-Neto, Antonio
[1]
;
Pontillo, Alessandra
[1]
;
Garcia Calich, Vera Lucia
[1]
;
Loures, Flavio Vieira
[1, 2]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Imunol, Sao Paulo - Brazil
[2] Univ Ferderal Sao Paulo, Inst Ciencia & Tecnol, Sao Jose Dos Campos - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | FRONTIERS IN IMMUNOLOGY; v. 9, MAR 20 2018. |
| Web of Science Citations: | 1 |
| Abstract | |
Plasmacytoid dendritic cells (pDCs), which have been extensively studied in the context of the immune response to viruses, have recently been implicated in host defense mechanisms against fungal infections. Nevertheless, the involvement of human pDCs during paracoccidioidomycosis (PCM), a fungal infection endemic to Latin America, has been scarcely studied. However, pDCs were found in the cutaneous lesions of PCM patients, and in pulmonary model of murine PCM these cells were shown to control disease severity. These findings led us to investigate the role of human pDCs in the innate phase of PCM. Moreover, considering our previous data on the engagement of diverse Toll-like receptors and C-type lectin receptors receptors in Paracoccidioides brasiliensis recognition, we decided to characterize the innate immune receptors involved in the interaction between human pDCs and yeast cells. Purified pDCs were obtained from peripheral blood mononuclear cells from healthy donors and they were stimulated with P. brasiliensis with or without blocking antibodies to innate immune receptors. Here we demonstrated that P. brasiliensis stimulation activates human pDCs that inhibit fungal growth and secrete pro-inflammatory cytokines and type I IFNs. Surprisingly, P. brasiliensis-stimulated pDCs produce mature IL-1 beta and activate caspase 1, possibly via inflammasome activation, which is a phenomenon not yet described during pDC engagement by microorganisms. Importantly, we also demonstrate that dectin-2 and dectin-3 are expressed on pDCs and appear to be involved (via Syk signaling) in the pDC-P. brasiliensis interaction. Moreover, P. brasiliensis-stimulated pDCs exhibited an efficient antigen presentation and were able to effectively activate CD4(+) and CD8(+) T cells. In conclusion, our study demonstrated for the first time that human pDCs are involved in P. brasiliensis recognition and may play an important role in the innate and adaptive immunity against this fungal pathogen. (AU) | |
| FAPESP's process: | 14/04783-2 - Study of the plasmacytoid and myeloid dendritic cells function during Paracoccidioides brasiliensis infection |
| Grantee: | Flávio Vieira Loures |
| Support Opportunities: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 17/00711-5 - Characterization of human pDCs in PCM: innate receptors expression and tolerance mechanisms |
| Grantee: | Nycolas Willian Preite |
| Support Opportunities: | Scholarships in Brazil - Scientific Initiation |
| FAPESP's process: | 16/23189-0 - Modulation of Treg/Th17 responses by Aryl Hydrocarbon Receptor Ligands in the Search for an Immunotherapeutic Procedure for Pulmonary Paracoccidioidomycosis |
| Grantee: | Vera Lucia Garcia Calich |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 13/02396-9 - The role of the NLRP3 inflammasome in pulmonary paracoccidioidomycosis |
| Grantee: | Claudia Feriotti |
| Support Opportunities: | Scholarships in Brazil - Post-Doctoral |