Draft genome sequence of an extensively drug-resistant Pseudomonas aeruginosa isolate belonging to ST644 isolated from a footpad infection in a Magellanic penguin (Spheniscus magellanicus)

Author(s)

Sellera, Fabio P. ^[1] ; Fernandes, Miriam R. ^[2] ; Moura, Quezia ^[3] ; Souza, Tiago A. ^[4] ; Nascimento, Cristiane L. ^[5] ; Cerdeira, Louise ^[2] ; Lincopan, Nilton ^{[3, 2]}

Affiliation

^[1] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Internal Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Clin Anal, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

^[4] Univ Sao Paulo, Inst Biomed Sci, Genome Invest & Anal Lab GENIAL, Sao Paulo - Brazil

^[5] Vet Unit Santos Aquarium, Santos - Brazil

• Document type: Journal article
• Source: JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE; v. 12, p. 88-89, MAR 2018.
• Web of Science Citations: 1

Abstract

Objectives: The incidence of multidrug-resistant bacteria in wildlife animals has been investigated to improve our knowledge of the spread of clinically relevant antimicrobial resistance genes. The aim of this study was to report the first draft genome sequence of an extensively drug-resistant (XDR) Pseudomonas aeruginosa ST644 isolate recovered from a Magellanic penguin with a footpad infection (bumblefoot) undergoing rehabilitation process. Methods: The genome was sequenced on an Illumina NextSeq (R) platform using 150-bp paired-end reads. De novo genome assembly was performed using Velvet v. 1.2.10, and the whole genome sequence was evaluated using bioinformatics approaches from the Center of Genomic Epidemiology, whereas an in-house method (mapping of raw whole genome sequence reads) was used to identify chromosomal point mutations. Results: The genome size was calculated at 6 436 450 bp, with 6357 protein-coding sequences and the presence of genes conferring resistance to aminoglycosides, beta-lactams, phenicols, sulphonamides, tetracyclines, quinolones and fosfomycin; in addition, mutations in the genes gyrA (Thr83Ile), parC (Ser87Leu), phoQ (Arg61His) and pmrB (Tyr345His), conferring resistance to quinolones and polymyxins, respectively, were confirmed. Conclusion: This draft genome sequence can provide useful information for comparative genomic analysis regarding the dissemination of clinically significant antibiotic resistance genes and XDR bacterial species at the human-animal interface. (C) 2017 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/08593-9 - Pan-Resistome of beta-lactamase (KPC-2, CTX-M-8, CTX-M-15)-producing Klebsiella pneumoniae and Escherichia coli isolates endemic in Brazil
• Grantee: Nilton Erbet Lincopan Huenuman
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/13527-2 - Ancestral relationship, pan-resistoma plasmid of Escherichia coli producing b-lactamases of extended spectrum (CTX-M) and of plasmid curing methods
• Grantee: Miriam Rodriguez Fernandes
• Support Opportunities: Scholarships in Brazil - Doctorate