| Full text | |
| Author(s): |
Nunes, Jethe O. F.
[1]
;
Apostolico, Juliana de Souza
[1]
;
Andrade, David A. G.
[1]
;
Ruiz, Francieli S.
[2]
;
Fernandes, Edgar R.
[1]
;
Andersen, Monica L.
[2]
;
Keller, Alexandre C.
[1, 3]
;
Rosa, Daniela S.
[1]
Total Authors: 8
|
| Affiliation: | [1] Fed Univ Sao Paulo UNIFESP EPM, Dept Microbiol Immunol & Parasitol, Rua Botucatu 862, 4 Andar, Sao Paulo - Brazil
[2] Fed Univ Sao Paulo UNIFESP EPM, Dept Psychobiol, Sao Paulo - Brazil
[3] Fed Univ Sao Paulo UNIFESP EPM, Dept Med, Div Nephrol, Sao Paulo - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Journal of Allergy and Clinical Immunology; v. 141, n. 3, p. 1018+, MAR 2018. |
| Web of Science Citations: | 4 |
| Abstract | |
Background: Although different studies associated sleep deprivation (SD) with systemic inflammatory changes, the effect of sleep duration on the pathology of allergic chronic diseases is poorly understood. Objective: We sought to evaluate the influence of SD on allergen-induced pulmonary inflammation. Methods: Ovalbumin (OVA)-sensitized C57BL/6 mice were exposed to a first set of intranasal OVA challenge under SD or healthy sleep (HS) conditions, followed by a second OVA challenge, 1 week apart. Some groups were subjected to corticosteroid treatment with dexamethasone. Results: OVA-sensitized mice with SD had more severe airway inflammation than the allergic group with HS. Analysis of lung parenchyma revealed that the inflammation in allergic mice with SD was marked by an influx of neutrophils (mainly) and eosinophils and secretion of IL-6, TNF-alpha, and IL-17 in contrast to the eosinophilic inflammation and IL-4 production observed in allergic mice with HS. The same cytokine profile was observed in ex vivo culture of cervical lymph node cells and splenocytes, indicating that in allergic mice SD favors immune responses toward a proinflammatory TH17 profile. This idea is supported by the fact that disruption of IL-17 signaling (IL-17 receptor A(-/-)) prevented airway neutrophilia in allergic mice with SD. Furthermore, allergic mice with SD became refractory to corticosteroid treatment in contrast to the allergic group with HS. Conclusion: Collectively, our data show that sleep quality participates in the progression of allergen-induced eosinophilic lung inflammation to corticosteroid-refractory neutrophilic manifestation. (AU) | |
| FAPESP's process: | 12/04692-1 - Th2 immune responses as a risk factor for focal segmental glomerulosclerosis |
| Grantee: | Alexandre de Castro Keller |
| Support Opportunities: | Regular Research Grants |
| FAPESP's process: | 14/15061-8 - In vivo targetting of HIV- CD4+ t cell epitopes to dendritic cells |
| Grantee: | Daniela Santoro Rosa |
| Support Opportunities: | Regular Research Grants |