In Vitro TyRP-1 Knockdown Based on siRNA Carried by Liquid Crystalline Nanodispersions: an Alternative Approach for Topical Treatment of Vitiligo

Author(s)

Tofani, Larissa Bueno ^[1] ; Depieri, Livia Vieira ^[1] ; Campos, Patricia Mazureki ^[1] ; Riul, Thalita Bachelli ^[1] ; Antonietto, Kamilla Swiech ^[1] ; de Abreu Fantini, Marcia Carvalho ^[2] ; Lopes Badra Bentley, Maria Vitoria ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Ave Cafe S-N, BR-14040903 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Phys Inst, Sao Paulo - Brazil

• Document type: Journal article
• Source: PHARMACEUTICAL RESEARCH; v. 35, n. 5 MAY 2018.
• Web of Science Citations: 1

Abstract

Purpose Vitiligo is a skin disease characterized by depigmentation and the presence of white patches that are associated with the loss of melanocytes. The most common explanation for the cause of this condition is that it is an autoimmune condition. TyRP-1 is involved in melanin pigment synthesis but can also function as a melanocyte differentiation antigen. This protein plays a role in the autoimmune destruction of melanocytes, which results in the depigmentation, characteristic of this disease. In this study, we evaluated liquid crystalline nanodispersions as non-viral vectors to deliver siRNA-TyRP-1 as an alternative for topical treatment of vitiligo. Methods Liquid crystalline nanodispersions were obtained and characterized with respect to their physical-chemical parameters including size, PdI and zeta potential, as well as Small Angle X-ray Scattering and complexing to siRNA. The effects of the liquid crystalline nanodispersions on the cellular viability, cell uptake and levels of the knockdown target TyRP-1 were evaluated in melan-A cells after 24 h of treatment. Results The liquid crystalline nanodispersions demonstrated adequate physical-chemical parameters including nanometer size and a PdI below 0.38. These systems promoted a high rate of cell uptake and an impressive TyRP-1 target knockdown (> 80%) associated with suitable loading of TyRp-1 siRNA. Conclusions We demonstrated that the liquid crystalline nanodispersions showed promising alternative for the topical treatment of vitiligo due to their physical parameters and ability in knockdown the target protein involved with autoimmune destruction of melanocytes. (AU)

• FAPESP's process: 14/50928-2 - INCT 2014: Pharmaceutical Nanotechnology: a transdisciplinary approach
• Grantee: Maria Vitória Lopes Badra Bentley
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 13/06559-0 - In vitro and in vivo evaluation of liquid-crystalline nandispersions to deliver siRNA-TRP-1 as an alternative for the topical treatment of vitiligo
• Grantee: Larissa Bueno Tofani
• Support Opportunities: Scholarships in Brazil - Master