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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monitoring cell line identity in collections of human induced pluripotent stem cells

Full text
Author(s):
Sarafian, Raquel [1] ; Morato-Marques, Mariana [1] ; Borsoi, Juliana [1] ; Pereira, Lygia Veiga [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Natl Lab Embryon Stem Cells LaNCE, Dept Genet & Evolutionary Biol, Biosci Inst, BR-05508900 Sao Paulo, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: STEM CELL RESEARCH; v. 28, p. 66-70, APR 2018.
Web of Science Citations: 0
Abstract

The ability to reprogram somatic cells into induced pluripotent stem cells (hiPSCs) has led to the generation of large collections of cell lines from thousands of individuals with specific phenotypes, many of which will be shared among different research groups as invaluable tools for biomedical research. As hiPSC-based research involves extensive culture of many cell lines, the issue periodic cell line identification is particularly important to ensure that cell line identity remains accurate. Here we analyzed the different commercially available genotyping methods considering ease of in-house genotyping, cost and informativeness, and applied one of them in our workflow for hiPSC generation. We show that the chosen STR method was able to establish a unique DNA profile for each of the 35 individuals/hiPSC lines at the examined sites, as well as identify two discrepancies resulting from inadvertently exchanged samples. Our results highlight the importance of hiPSC line genotyping by an in-house method that allows periodic cell line identification and demonstrate that STR is a useful approach to supplement less frequent karyotyping and epigenetic evaluations. (c) 2018 The Authors. Published by Elsevier B.V. (AU)

FAPESP's process: 16/16076-4 - Functional analysis of FBN1 mutations in human Pluripotent Stem-Cells (hPSCs)
Grantee:Juliana Borsoi Sant'Ana
Support type: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 16/20650-8 - Assessment of hIPSC- derived hepatocytes as a model to study the responsiveness losartan in vitro
Grantee:Raquel Delgado Sarafian
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC