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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effect of Resistance Training on Extracellular Matrix Adaptations in Skeletal Muscle of Older Rats

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Author(s):
Guzzoni, Vinicius [1] ; Ribeiro, Manoel B. T. [2] ; Lopes, Gisele N. [3] ; Marqueti, Rita de Cassia [4] ; de Andrade, Rosangela V. [5] ; Selistre-de-Araujo, Heloisa S. [3] ; Durigan, Joao L. Q. [4]
Total Authors: 7
Affiliation:
[1] Univ Brasilia, Brasilia, DF - Brazil
[2] Univ Brasilia, Dept Phys Educ, Brasilia, DF - Brazil
[3] Univ Fed Sao Carlos, Ctr Biol & Hlth Sci, Dept Physiol Sci, Sao Carlos, SP - Brazil
[4] Univ Brasilia, Grad Program Rehabil Sci, Brasilia, DF - Brazil
[5] Univ Catolica Brasilia, Grad Program Genom & Prote, Brasilia, DF - Brazil
Total Affiliations: 5
Document type: Journal article
Source: FRONTIERS IN PHYSIOLOGY; v. 9, APR 11 2018.
Web of Science Citations: 4
Abstract

Accumulation of connective tissue, particularly extracellular matrix (ECM) proteins, has been observed in skeletal muscles with advancing age. Resistance training (RT) has been widely recommended to attenuate age-induced sarcopenia, even though its effects on the components that control ECM turnover in skeletal muscles remain to be elucidated. Thus, the aim of this study was to determine the effects of RT on connective tissue content and gene expression of key components of ECM in the skeletal muscles of aged rats. Young (3 mo.) and older (21 mo.) adult male Wistar rats were submitted to a RT protocol (ladder climbing with 65, 85, 95, and 100% load), 3 times a week for 12 weeks. Forty-eight hours post-training, the soleus (SOL) and gastrocnemius (GAS) muscles were dissected for histological and mRNA analysis. RT mitigated the age-associated increase of connective tissue content in both muscles, even though mRNA levels of COL-1 and-3 were elevated in older trained rats. Overall, RT significantly elevated the gene expression of key components of connective tissue deposition (TGF beta and CTGF; MMP-2 and-9; TIMP-1 and-2) in the GAS and SOL muscles of older rats. In conclusion, RT blunted the age-induced accumulation of connective tissue concomitant to the upregulation of genes related to synthesis and degradation of the ECM network in the SOL and GAS muscles of older rats. Although our findings indicate that RT plays a crucial role reducing connective tissue accumulation in aged hindlimb muscles, key components of ECM turnover were paradoxically elevated. The phenotypic responses induced by RT were not accompanied by the gene expression of those components related to ECM turnover. (AU)

FAPESP's process: 11/11229-3 - Aging-related alterations in extracellular matrix and its adaptations to strength training in calcaneal tendon of rats: molecular, cellular, and biomechanical approach
Grantee:Heloisa Sobreiro Selistre de Araújo
Support Opportunities: Regular Research Grants
FAPESP's process: 13/00798-2 - The extracellular matrix in aging, exercise and in the tumor microenvironment
Grantee:Heloisa Sobreiro Selistre de Araújo
Support Opportunities: Research Projects - Thematic Grants