Study of epigenetic regulation of the embryonic gene, PAX3, by diabetic pregnancy-...
| Full text | |
| Author(s): |
Bomfim, Monalisa M.
[1]
;
Andrade, Gabriella M.
[1]
;
del Collado, Maite
[1]
;
Sangalli, Juliano R.
[1]
;
Fontes, Patricia K.
[2]
;
Nogueira, Marcelo F. G.
[3]
;
Meirelles, Flavio V.
[1]
;
da Silveira, Juliano C.
[1]
;
Perecin, Felipe
[1]
Total Authors: 9
|
| Affiliation: | [1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Ave Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
[3] Sao Paulo State Univ, Sch Sci Humanities & Languages, Dept Biol Sci, Assis, SP - Brazil
Total Affiliations: 3
|
| Document type: | Journal article |
| Source: | Molecular Reproduction and Development; v. 84, n. 12, p. 1296-1305, DEC 2017. |
| Web of Science Citations: | 6 |
| Abstract | |
Early mammalian embryos derived from in vitro fertilization are exposed to conditions distinct from the native oviduct-uterine environment, including atmospheric oxygen that promotes cellular oxidative stress and alters gene expression. High oxygen partial pressure during embryo development is associated with low pregnancy rates and increased embryonic apoptosis. We investigated how bovine embryos responded to high (20%) or low (5%) oxygen partial pressure during in vitro culture, evaluating levels of reactive oxygen species (ROS) as well as changes in the expression of oxidative stress- and epigenetic-related transcripts and miRNAs in blastocysts. Additionally, we determined the global DNA methylation levels in the resulting embryos. Our data indicated that bovine blastocysts produced in vitro under high oxygen partial pressure possessed elevated ROS abundance and exhibited increased expression of CAT, GLRX2, KEAP1, NFR2, PRDX1, PRDX3, SOD1, TXN, and TXNRD1, versus reduced levels of the oxidative stress-related bta-miR-210. These stressed embryos also presented altered expression of the epigenetic-associated transcripts DNMT3A, H2AFZ, H3F3B, HDAC2, MORF4L2, REST, and PAF1. In addition, we demonstrated that embryos cultured under high oxygen partial pressure have increased global DNA methylation, suggesting that DNA hypermethylation is mediated by the deregulation of epigenetic-related enzymes due to oxidative stress. (AU) | |
| FAPESP's process: | 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation |
| Grantee: | Felipe Perecin |
| Support type: | Regular Research Grants |
| FAPESP's process: | 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos |
| Grantee: | Juliano Coelho da Silveira |
| Support type: | Research Grants - Young Investigators Grants |
| FAPESP's process: | 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle |
| Grantee: | Marcelo Fábio Gouveia Nogueira |
| Support type: | Research Projects - Thematic Grants |
| FAPESP's process: | 14/21042-6 - Effects of oxidative stress during bovine in vitro embryo production on miR199a and its target genes ERBB2 and ERBB3 |
| Grantee: | Monalisa Medrado Bomfim |
| Support type: | Scholarships in Brazil - Master |
| FAPESP's process: | 13/08135-2 - CTC - Center for Cell-Based Therapy |
| Grantee: | Dimas Tadeu Covas |
| Support type: | Research Grants - Research, Innovation and Dissemination Centers - RIDC |