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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Antioxidant responses and deregulation of epigenetic writers and erasers link oxidative stress and DNA methylation in bovine blastocysts

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Author(s):
Bomfim, Monalisa M. [1] ; Andrade, Gabriella M. [1] ; del Collado, Maite [1] ; Sangalli, Juliano R. [1] ; Fontes, Patricia K. [2] ; Nogueira, Marcelo F. G. [3] ; Meirelles, Flavio V. [1] ; da Silveira, Juliano C. [1] ; Perecin, Felipe [1]
Total Authors: 9
Affiliation:
[1] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, Ave Duque Caxias Norte 225, BR-13635900 Pirassununga, SP - Brazil
[2] Sao Paulo State Univ, Inst Biosci, Dept Pharmacol, Botucatu, SP - Brazil
[3] Sao Paulo State Univ, Sch Sci Humanities & Languages, Dept Biol Sci, Assis, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Molecular Reproduction and Development; v. 84, n. 12, p. 1296-1305, DEC 2017.
Web of Science Citations: 5
Abstract

Early mammalian embryos derived from in vitro fertilization are exposed to conditions distinct from the native oviduct-uterine environment, including atmospheric oxygen that promotes cellular oxidative stress and alters gene expression. High oxygen partial pressure during embryo development is associated with low pregnancy rates and increased embryonic apoptosis. We investigated how bovine embryos responded to high (20%) or low (5%) oxygen partial pressure during in vitro culture, evaluating levels of reactive oxygen species (ROS) as well as changes in the expression of oxidative stress- and epigenetic-related transcripts and miRNAs in blastocysts. Additionally, we determined the global DNA methylation levels in the resulting embryos. Our data indicated that bovine blastocysts produced in vitro under high oxygen partial pressure possessed elevated ROS abundance and exhibited increased expression of CAT, GLRX2, KEAP1, NFR2, PRDX1, PRDX3, SOD1, TXN, and TXNRD1, versus reduced levels of the oxidative stress-related bta-miR-210. These stressed embryos also presented altered expression of the epigenetic-associated transcripts DNMT3A, H2AFZ, H3F3B, HDAC2, MORF4L2, REST, and PAF1. In addition, we demonstrated that embryos cultured under high oxygen partial pressure have increased global DNA methylation, suggesting that DNA hypermethylation is mediated by the deregulation of epigenetic-related enzymes due to oxidative stress. (AU)

FAPESP's process: 14/21034-3 - Intra and intercellular routes during foliculogenesis and in vivo and in vitro oocyte maturation
Grantee:Felipe Perecin
Support type: Regular Research Grants
FAPESP's process: 14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos
Grantee:Juliano Coelho da Silveira
Support type: Research Grants - Young Investigators Grants
FAPESP's process: 12/50533-2 - GIFT: genomic improvement of fertilization traits in Danish and Brazilian cattle
Grantee:Marcelo Fábio Gouveia Nogueira
Support type: Research Projects - Thematic Grants
FAPESP's process: 14/21042-6 - Effects of oxidative stress during bovine in vitro embryo production on miR199a and its target genes ERBB2 and ERBB3
Grantee:Monalisa Medrado Bomfim
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/08135-2 - CTC - Center for Cell-Based Therapy
Grantee:Dimas Tadeu Covas
Support type: Research Grants - Research, Innovation and Dissemination Centers - RIDC