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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Uphill Running Excessive Training Increases Gastrocnemius Glycogen Content in C57BL/6 Mice

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Author(s):
Morais, G. P. [1] ; da Rocha, A. [2] ; Pinto, A. P. [2] ; Oliveira, L. da C. [2] ; de Vicente, L. G. [2] ; Ferreira, G. N. [1] ; de Freitas, E. C. [1] ; da Silva, A. S. R. [2, 1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Postgrad Program Phys Educ & Sport, Ribeirao Preto - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Physiological Research; v. 67, n. 1, p. 107-115, 2018.
Web of Science Citations: 0
Abstract

The main aim of the present investigation was to verify the effects of three overtraining (OT) protocols performed in downhill (OTR/down), uphill (OTR/up) and without inclination (OTR) on the protein levels of Akt (Ser473), AMPK alpha (Thr172), PGC-1 alpha, plasma membrane GLUT-1 and GLUT-4 as well as on the glycogen contents in mice gastrocnemius. A trained (TR) protocol was used as positive control. Rodents were divided into naive (N, sedentary mice), control (CT, sedentary mice submitted to the performance evaluations), TR, OTR/down, OTR/up and OTR groups. At the end of the experimental protocols, gastrocnemius samples were removed and used for immunoblotting analysis as well as for glycogen measurements. There was no significant difference between the experimental groups for the protein levels of pAkt (Ser473), pAMPK alpha (Thr172), PGC-1 alpha, plasma membrane GLUT-1 and GLUT-4. However, the OTR/up protocol exhibited higher contents of glycogen compared to the CT and TR groups. In summary, the OTR/up group increased the gastrocnemius glycogen content without significant changes of pAkt (Ser473), pAMPK alpha (Thr172), PGC-1 alpha, plasma membrane GLUT-1 and GLUT-4. (AU)

FAPESP's process: 14/25459-9 - Nonfunctional overreaching in animal model: inflammatory and hypertrophic adaptions of the cardiac muscle
Grantee:Alisson Luiz da Rocha
Support type: Scholarships in Brazil - Master
FAPESP's process: 13/20591-3 - Responses of proteins from the inflammatory, insulinic and hypertrophic molecular pathways to nonfunctional overreaching induced by treadmill running performed in downhill, without inclination and uphill in skeletal muscle of mice
Grantee:Adelino Sanchez Ramos da Silva
Support type: Regular Research Grants
FAPESP's process: 15/08013-0 - Overreaching not functional in animal model and endoplasmic reticulum stress in muscle heart and liver
Grantee:Ana Paula Pinto
Support type: Scholarships in Brazil - Master