Effect of rosmarinic acid on the arterial blood pressure in normotensive and hypertensive rats: Role of ACE

Author(s)

Ferreira, Luciana Garros ^[1] ; Barbosa Evora, Paulo Roberto ^[1] ; Capellini, Verena Kise ^[1] ; Albuquerque, Agnes Afrodite ^[1] ; Menezes Carvalho, Marco Tulio ^[1] ; da Silva Gomes, Roseli Aparecida ^[2] ; Parolini, Marco Tulio ^[2] ; Celotto, Andrea Carla ^{[3, 1]}

Affiliation

^[1] Univ Sao Paulo, Ribeirao Preto Fac Med, Dept Surg & Anat, Postgrad Program Med Surg Clin, Ribeirao Preto, SP - Brazil

^[2] Univ Triangulo Mineiro, Inst Biol & Nat Sci, Uberaba, MG - Brazil

^[3] Dr Paulo Prata FACISB, Barretos Sch Hlth Sci, Barretos, SP - Brazil

• Document type: Journal article
• Source: Phytomedicine; v. 38, p. 158-165, JAN 1 2018.
• Web of Science Citations: 6

Abstract

Background: In recent years, it has been demonstrated the inhibitory effect of some plant species on the angiotensin-converting enzyme and rosmarinic acid is a prominent constituent of these species. Hypothesis/Purpose: This study was carried out to verify the effect of rosmarinic acid on blood pressure through inhibitory activity on angiotensin-converting enzyme in rats. Study design: The arterial hypertension was promoted using 2-kidneys 1-clip model in rats. The potential inhibitory rosmarinic acid effect on angiotensin-converting enzyme activity was compared with captopril actions by analyzing in vivo blood pressure dose-response curves to angiotensin I and bradykinin. The in vitro plasma angiotensin-converting enzyme activity was measured by fluorimetry using the substrate Abz-FRK(Dnp) P-OH substrate. In addition, dosages of nitrite/nitrate analysis were carried out. Results: (1) rosmarinic acid caused systolic blood pressure dose-dependent decrease in hypertensive rats; (2) The angiotensin I dose-response curves demonstrated that rosmarinic acid promotes minor changes in systolic blood pressure only in the hypertensive group; (3) The bradykinin dose-response curves showed that both rosmarinic acid and captopril promoted a systolic blood pressure reduction, but only the captopril effect was significant; (4) The angiotensin-converting enzyme activity in rat lung tissue was inhibited by the rosmarinic acid in a dose dependent manner; (5) The analysis of nitrite/nitrate plasma concentrations showed no significant difference among the experimental groups. Conclusion: The rosmarinic acid is effective in reducing blood pressure, selectively, only in hypertensive animals. The rosmarinic acid (173 mu M) promoted almost a 98.96% reduction on angiotensin-converting enzyme activity. (C) 2017 Elsevier GmbH. All rights reserved. (AU)

• FAPESP's process: 09/03030-2 - Effect of rosmarinic acid on angiotensin converting-enzyme inhibition in normotensive and hypertensive rats.
• Grantee: Luciana Garros Ferreira
• Support Opportunities: Scholarships in Brazil - Master