Enhanced immunization techniques to obtain highly specific monoclonal antibodies

Author(s):Show less -

de Almeida, Rodrigo ^[1] ; Nakamura, Cecilia Naomi ^[1] ; de Lima Fontes, Marina ^[1] ; Deffune, Elenice ^[2] ; Felisbino, Sergio Luis ^[3] ; Kaneno, Ramon ^[4] ; Favaro, Wagner Jose ^[5] ; Billis, Athanase ^[6] ; Cerri, Marcel Otavio ^[7] ; Fusco-Almeida, Ana Marisa ^[1] ; Mendes Giannini, Maria Jose ^[1] ; Moroz, Andrei ^{[1, 7, 2]}

Affiliation

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Prote Ctr, Monoclonal Antibody Lab, UNESP, Araraquara - Brazil

^[2] Univ Estadual Paulista, Fac Med, Blood Transfus Ctr, Cell Engn Lab, UNESP, Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias, Dept Morphol, Extracellular Matrix Lab, UNESP, Botucatu, SP - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Immunol, Tumor Immunol Lab, UNESP, Botucatu, SP - Brazil

^[5] Univ Campinas UNICAMP, Biol Inst, Dept Struct & Funct Biol, Immunotherapy Lab, Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Sch Med, Dept Pathol, UNICAMP, Uropathol Lab, Campinas, SP - Brazil

^[7] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Bioproc & Biotechnol, UNESP, Araraquara - Brazil

• Document type: Review article
• Source: mAbs; v. 10, n. 1, p. 46-54, 2018.
• Web of Science Citations: 2

Abstract

Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms: subtractive immunization, monoclonal antibody, tolerization, neonatal, high-zone tolerance, masking immunization. Patent records were also consulted. From the list of results, we included all available reports, from 1985 to present, that used any enhanced immunization technique to produce either polyclonal or monoclonal antibodies. Our examination yielded direct evidence that these enhanced immunization techniques are efficient in obtaining specific antibodies to rare epitopes, with different applications, such as to identify food contaminants or tumor cells. (AU)

• FAPESP's process: 15/21884-0 - Production of murine monoclonal antibodies recognizing prostate tumor cells
• Grantee: Rodrigo de Almeida
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 14/23738-8 - Production of murine monoclonal antibodies against metastasis-associated antigens of prostate cancer cells using subtractive immunization
• Grantee: Andrei Moroz
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/07047-0 - Subtractive immunization efficacy on production of antibodies specific to prostate tumor cells
• Grantee: Cecília Naomi Nakamura
• Support Opportunities: Scholarships in Brazil - Scientific Initiation