Advanced search
Start date
Betweenand
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Effects of high-impact exercise on the physical properties of bones of ovariectomized rats fed to a high-protein diet

Full text
Author(s):
Shimano, R. C. [1] ; Yanagihara, G. R. [1] ; Macedo, A. P. [1] ; Yamanaka, J. S. [1] ; Shimano, A. C. [1] ; Tavares, J. M. R. S. [2] ; Issa, J. P. M. [3]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biomecan Med & Reabil Aparelho, Sao Paulo - Brazil
[2] Univ Porto, Fac Engn, Dept Engn Mecan, Inst Ciencia & Inovacao Engn Mecan & Engn Ind, Porto - Portugal
[3] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Dept Morfol Fisiol & Patol Basica, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS; v. 28, n. 5, p. 1523-1531, MAY 2018.
Web of Science Citations: 1
Abstract

The aim of this study was to evaluate the effects of high-impact physical exercise as a prophylactic and therapeutic means in osteopenic bones of rats submitted to ovariectomy and protein diet intake. A total of 64 Wistar rats were divided into eight groups (n=8 each), being: OVX, ovx, standard diet and sedentary; OVXE, ovx, standard diet and jump; OVXP, ovx, high-protein diet and sedentary; and OVXEP, ovx, high-protein diet and jump; SH, sham, standard diet and sedentary; SHE, sham, standard diet and jump; SHP, sham, high-protein diet and sedentary; and SHEP, sham, high-protein diet and jump. OVX surgery consists of ovariectomy, and sham was the control surgery. The jumping protocol consisted of 20 jumps/day, 5days/week. The bone structure was evaluated by densitometry, mechanical tests, histomorphometric, and immunohistochemical analyses. A high-protein diet resulted in increased bone mineral density (P=.049), but decreased maximal load (P=.026) and bone volume fraction (P=.023). The benefits of physical exercise were demonstrated by higher values of the maximal load in the trained groups compared to the sedentary groups (P<.001). The sham groups had decreased immunostaining of osteocalcin (P=.004) and osteopontin (P=.010) compared to ovx groups. However, the high-protein diet (P=.005) and jump exercise (P=.017) resulted in lower immunostaining of osteopontin compared to the standard diet and sedentary groups, respectively. In this experimental model, it was concluded that ovariectomy and a high-fat diet can negatively affect bone tissue and the high-impact exercise was not enough to suppress the deleterious effects caused by the protein diet and ovariectomy. (AU)

FAPESP's process: 14/10733-8 - Tissue and biomechanics analysis of bones of rats fed high-fat diet: experimental study of the relationship between fat mass and bone loss
Grantee:Antônio Carlos Shimano
Support type: Regular Research Grants
FAPESP's process: 14/25879-8 - Tissue and biomechanical analysis of bones of ovariectomized rats fed a high-fat diet
Grantee:Gabriela Rezende Yanagihara
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 15/16194-4 - The role of MMP-9 in the pathogenesis of osteoporosis in animal models of atherosclerosis
Grantee:João Paulo Mardegan Issa
Support type: Regular Research Grants
FAPESP's process: 15/26142-1 - Image Processing and Analysis obtained by Scanning Electron Microscopy of bone of female obese and eutrophic rats
Grantee:Gabriela Rezende Yanagihara
Support type: Scholarships abroad - Research Internship - Doctorate