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Image Processing and Analysis obtained by Scanning Electron Microscopy of bone of female obese and eutrophic rats

Grant number: 15/26142-1
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): May 02, 2016
Effective date (End): August 02, 2016
Field of knowledge:Engineering - Biomedical Engineering
Principal researcher:Antônio Carlos Shimano
Grantee:Gabriela Rezende Yanagihara
Supervisor abroad: Joao Manuel Ribeiro da Silva Tavares
Home Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Research place: Universidade do Porto (UP), Portugal  
Associated to the scholarship:14/25879-8 - Tissue and biomechanical analysis of bones of ovariectomized rats fed a high-fat diet, BP.DR


One of the major areas of Engineering is the area of Image Processing and Image Analysis. For the community is extremely important, because techniques and methods of processing and image analysis are used in different fields, one of which is the area of health. Images often used in experimental research related to biomedicine are those obtained for scanning electron microscopic(SEM). However, for the biological tissue images, more specifically to bone, there is an effective and reliable means of analysis, not be purely qualitative. While providing 2D images, the depth of field that the SEM provides gives the images a three-dimensional look that must be taken into account, particularly when you want to assess the microarchitecture and bone quality. The aim of the research project to be developed during the internship abroad is working with technical and computer processing methods and analysis of images obtained by SEM of obese rats and eutrophic, belonging to the research project of the doctoral thesis. Following the acquisition of the images, they will be processed to improve and enhance subjectively to subsequently adopt a method of analysis that takes into account the three-dimensional appearance of images. This would also try to get a way to quantify important parameters in the evaluation of bone quality.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SHIMANO, R. C.; YANAGIHARA, G. R.; MACEDO, A. P.; YAMANAKA, J. S.; SHIMANO, A. C.; TAVARES, J. M. R. S.; ISSA, J. P. M. Effects of high-impact exercise on the physical properties of bones of ovariectomized rats fed to a high-protein diet. SCANDINAVIAN JOURNAL OF MEDICINE & SCIENCE IN SPORTS, v. 28, n. 5, p. 1523-1531, MAY 2018. Web of Science Citations: 1.
REZENDE YANAGIHARA, G.; CARMINATI SHIMANO, R.; ATSUKO TIDA, J.; SUZUKI YAMANAKA, J.; YASUYO FUKADA, S.; MARDEGAN ISSA, J. P.; SHIMANO, A. C.; TAVARES, JOAO MANUEL R. S. Influence of high-fat diet on bone tissue: An experimental study in growing rats. Journal of Nutrition Health & Aging, v. 21, n. 10, p. 1337-1343, DEC 2017. Web of Science Citations: 2.

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