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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

In vitro regeneration from leaf-base segments in three genotypes of Urochloa spp.

Full text
Author(s):
Yaguinuma, Diliane Harumi [1] ; Takamori, Luciana Midori [1] ; de Oliveira, Adriana Mendonca [1] ; Esteves Vieira, Luiz Gonzaga [1] ; Ribas, Alessandra Ferreira [1]
Total Authors: 5
Affiliation:
[1] Univ Oeste Paulista, Fac Agron, BR-19067175 Presidente Prudente - Brazil
Total Affiliations: 1
Document type: Journal article
Source: CROP & PASTURE SCIENCE; v. 69, n. 5, p. 527-534, 2018.
Web of Science Citations: 1
Abstract

The key agricultural species of Urochloa P.Beauv. (signal grass), important as tropical forage grasses, are characterised by asexual seed formation (apomixis), and this presents a challenge for breeding programs. Biotechnological approaches could be an option to develop improved cultivars. We evaluated the regenerative potential from three commercial genotypes, U. brizantha cv. Marandu, U. decumbens cv. Basilisk and U. ruziziensis cv. Ruziziensis, by using leaf-base segments as explants. We tested two auxins (2,4-D and picloram) and one cytokinin (TDZ) at four concentrations (1, 2, 3 and 4 mg L-1). Seeds were scarified, peeled and disinfected before inoculation on half-strength MS media in the dark for 14 days. Leaf-base explants were sectioned in thin slices and inoculated into the media. We analysed the number of primary calluses, number of calluses with shoots clusters and the average of regenerated plants. The lowest concentration of auxins tested (1 mg L-1) yielded the highest number of regenerated plants for Marandu and Basilisk, whereas the optimum for Ruziziensis was 2 mg L-1. Medium with higher concentrations of TDZ (4 mg L-1) was required to produce high frequency of plants for all genotypes. Explants cultured on media with TDZ produced very few calluses. These results indicate that the auxins and cytokinin tested can induce plant regeneration from Urochloa leaf-base segments, and may be used to produce transgenic plants in genetic transformation studies. (AU)

FAPESP's process: 13/04819-4 - Genetic transformation of Urochloa brizantha for improving abiotic stress tolerance
Grantee:Alessandra Ferreira Ribas
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 16/06874-0 - AIPC cassette:ipt construction and establishment of co-bombardment conditions
Grantee:Diliane Harumi Yaguinuma
Support Opportunities: Scholarships in Brazil - Scientific Initiation