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Genetic transformation of Urochloa brizantha for improving abiotic stress tolerance


In Brazil, raising cattle for meat and milk production is done in approximately 172 million hectares, in which grasses from the genus Urochloa represent the major cultivated species. In São Paulo, the area occupied with pastures is 39% from total and the genus Urochloa represents 89%, being the species U. brizantha the most used. Despite of their high biomass yield in the tropics, species from Urochloa genus are subjected to environmental stresses. Hence, the search of plants that can growth in sub-optimal conditions is crucial for maintaining the sustainability in these production systems. U. brizantha cultivars are clones due to their apomitic reproduction system, and therefore can be considered monocultures that implies into great economic and environmental risk. As a result, for reaching their production and growth potentials it is necessary to have genotypes with increased tolerance to adverse factors such water deficit and cold stress. Genetic modified plants expressing genes that codes for the key enzyme in the biosynthesis of proline (1-pirrolina-5-carboxilato sintetase - P5CS) or for the first enzyme involved in the synthesis of the growth regulator citokinin (isopenteniltransferase - ipt) have been very effective in increasing the tolerance to abiotic stress. Thus, this study aims to transform U. brizantha with the P5CS and ipt genes under the control of stress inducible promoters AIPC (ABA inducible promoter complex) and COR15 (cold-related promoter), and evaluating the transformed plants for tolerance to abiotic stress. (AU)

Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
YAGUINUMA, DILIANE HARUMI; TAKAMORI, LUCIANA MIDORI; DE OLIVEIRA, ADRIANA MENDONCA; ESTEVES VIEIRA, LUIZ GONZAGA; RIBAS, ALESSANDRA FERREIRA. In vitro regeneration from leaf-base segments in three genotypes of Urochloa spp.. CROP & PASTURE SCIENCE, v. 69, n. 5, p. 527-534, 2018. Web of Science Citations: 1.
TAKAMORI, LUCIANA MIDORI; CARRION PEREIRA, ALYNE VALERIA; SOUZA, GUSTAVO MAIA; ESTEVES VIEIRA, LUIZ GONZAGA; RIBAS, ALESSANDRA FERREIRA. Identification of Endogenous Reference Genes for RT-qPCR Expression Analysis in Urochloa brizantha Under Abiotic Stresses. SCIENTIFIC REPORTS, v. 7, AUG 17 2017. Web of Science Citations: 3.
TAKAMORI, LUCIANA MIDORI; MACHADO NETO, NELSON BARBOSA; ESTEVES VIEIRA, LUIZ GONZAGA; RIBAS, ALESSANDRA FERREIRA. Optimization of somatic embryogenesis and in vitro plant regeneration of Urochloa species using picloram. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, v. 51, n. 5, p. 554-563, OCT 2015. Web of Science Citations: 6.

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