Selective and sensitive voltammetric determination of folic acid using graphite/restricted access molecularly imprinted poly(methacrylic acid)/SiO2 composite

Author(s)

Kuceki, Michele ^[1] ; de Oliveira, Fernanda Midori ^[1] ; Segatelli, Mariana Gava ^[1] ; Lombello Coelho, Malena Karla ^[2] ; Pereira, Arnaldo Cesar ^[2] ; da Rocha, Luana Rianne ^[1] ; Mendonca, Jhessica de Cassia ^[1] ; Teixeira Tarley, Cesar Ricardo ^{[1, 3]}

Affiliation

^[1] Univ Estadual Londrina, Dept Quim, Rodovia Celso Garcia Cid, PR 445, Km 380, BR-86050482 Londrina, PR - Brazil

^[2] Univ Fed Sao Joao Rei UFSJ, Dept Ciencias Nat, Campus Dom Bosco, Praca Dom Helvecio 74, BR-36301160 Sao Joao Del Rei, MG - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Nacl Ciencia & Tecnol Bioanalit INCTBio, Inst Quim, Dept Quim Analit, Cidade Univ Zeferino Vaz Campus S-N, BR-13083861 Campinas, SP - Brazil

• Document type: Journal article
• Source: JOURNAL OF ELECTROANALYTICAL CHEMISTRY; v. 818, p. 223-230, JUN 1 2018.
• Web of Science Citations: 4

Abstract

The present study demonstrates the development of a voltammetric sensor based on graphite/restricted access molecularly imprinted poly(methacrylic acid)/SiO2 composite for folic acid determination in pharmaceutical formulation. The insertion of hybrid molecularly imprinted polymer with restricted access (RAM-MIP) into the graphite paste provided enhance in the cathodic peak current for FA. As the electric conductivity of RAM-MIP is very poor, the optimum content was found to be very low (3%, w/w) in the composite. Under optimized conditions (0.1 mol L-1 Britton-Robinson buffer and pH 4.5) using square wave voltammetry (frequency of 150 Hz, pulse amplitude of 90 mV and potential increment of 7 mV) a limit of detection of 0.72 mu g L-1 (1.63 nmol L-1) and a linear range from 5.0 to 100.0 mu g L-1 (0.01 to 0.23 mu mol L-1) were obtained. The method precision was assessed in terms of inter-day (n = 6) and infra-day precision (two consecutive days), whose relative standard deviations (RSD%) ranged from 3.56 to 5.01%. The developed method was applied for FA determination in pharmaceutical samples whose accuracy was attested by means of addition and recovery assays (97.7-105.0%) as well as by high performance liquid chromatography (HPLC) as a reference technique, thus indicating that RAM-MIP sensor might be successfully applied for quality control in the real samples. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants