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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Menopause transition promotes distinct modulation of mRNAs and miRNAs expression in calvaria and bone marrow osteoblastic cells

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Semeghini, Mayara Sgarbi [1] ; de Azevedo, Fernanda Grilo [1] ; Fernandes, Roger Rodrigo [1] ; Assis, Amanda Freire [2] ; Dernowsek, Janaina Andrea [2] ; Rosa, Adalberto Luiz [3] ; Siessere, Selma [1] ; Passos, Geraldo Aleixo [1, 2] ; Bombonato-Prado, Karina Fittipaldi [1]
Total Authors: 9
[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Morphol Physiol & Basic Pathol, Ribeirao Preto, SP - Brazil
[2] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Mol Immunogenet Grp, Ribeirao Preto, SP - Brazil
[3] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Oral & Maxillofacial Surg & Periodontol, Ribeirao Preto, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Cell Biology International; v. 42, n. 1, p. 12-24, JAN 2018.
Web of Science Citations: 3

Investigation on functional genome research may contribute to the knowledge of functional roles of different mRNAs and miRNAs in bone cells of osteoporotic animals. Currently, few studies indicate the changes in gene modulation that osteoporosis causes in osteoblastic cells from different sites. Thus, the purpose of this investigation was to evaluate cell viability, alkaline phosphatase activity and modulation of mRNAs/miRNAs in osteoblastic cells from calvaria and bone marrow by means of microarray technology. Wistar female rats were divided in sham operated and ovariectomized groups. After 150 days of ovariectomy, cells were isolated from both sites to perform cell culture. Results showed that calvaria cells from ovariectomized rats had a decrease in viability when compared to control groups and to bone marrow cells from osteoporotic rats after 3 days. Alkaline phosphatase activity decreased in calvaria cells from ovariectomized rats whereas it was increased in bone marrow osteoblastic cells in the same group. Microarray data analysis showed 5447 differentially expressed mRNAs and 82 differentially expressed miRNAs in calvaria cells. The same way, 4399 mRNAs and 54 miRNAs were expressed in bone marrow cells. mRNAs associated with bone metabolism such as Anxa5, Sp7, Spp1, Notch1 were distinctively modulated in both sites, as well as miRNAs such as miR-350, miR-542-3p, miR-204-5p, and miR-30e-3p. The RNA species identified in this study could be further used as targets for treatment or prevention of osteoporosis. (AU)

FAPESP's process: 12/15889-0 - Large scale gene expression evaluation of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.
Grantee:Mayara Sgarbi Semeghini
Support type: Scholarships in Brazil - Master
FAPESP's process: 12/17747-9 - Evaluation of osteogenesis and transcriptional profiles (mRNA and microRNAs) of osteoblastic cells from calvaria and bone marrow of female rats submitted to osteoporosis
Grantee:Karina Fittipaldi Bombonato Prado
Support type: Regular Research Grants