Large scale gene expression evaluation of osteoblastic cells from calvaria and bone marrow of ovariectomized rats.

• Grant number: 12/15889-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: November 01, 2012
• End date: August 31, 2014
• Field of knowledge: Biological Sciences - Morphology - Cytology and Cell Biology
• Principal Investigator: Karina Fittipaldi Bombonato Prado
• Grantee: Mayara Sgarbi Semeghini
• Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Currently, the mesenchymal cells of different origins are considered sources for replacement tissues, these cells have osteogenic potential and may be eligible for repair and maintenance of bone tissue and are very attractive for tissue engineering. However, changes in behavior can be caused by factors like osteoporosis, affecting their ability to self-renewal and differentiation. Therefore, the objective of this project is to compare the gene expression of differentiated mesenchymal cells into osteoblastic cells from bone marrow with osteoblastic cells already differentiated from the calvaria. There will be used 18 Wistar rats divided into control and treated groups (induction of osteoporosis). The rats in the treated group will be submitted to bilateral ovariectomy through the appropriate protocol and 150 days after surgery, the rats of both groups will be sacrificed to collect the femurs and fragments of calvaria. From the collected materials the mesenchymal cells from bone marrow and osteoblast cells of calvaria will be isolated and placed in culture bottles with supplemented culture media. After confluence, the RNA will be extracted to performed the identification of induced and repressed genes in cells of control and osteoporosis rats using Agilent blades for microarray and miRNA (n = 3). Data will be analyzed with the aid of specialized bioinformatics programs like SAM (Significance analysis of microarrays), Cluster-TreeView and GeneNetwork and GeneSpring bioinformatics platform (Agilent). From these data of levels of transcription we will rebuild gene interaction networks to finally evaluate in conjunction the role of each in response to osteoporosis.

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

SEMEGHINI, MAYARA SGARBI; DE AZEVEDO, FERNANDA GRILO; FERNANDES, ROGER RODRIGO; ASSIS, AMANDA FREIRE; DERNOWSEK, JANAINA ANDREA; ROSA, ADALBERTO LUIZ; SIESSERE, SELMA; PASSOS, GERALDO ALEIXO; BOMBONATO-PRADO, KARINA FITTIPALDI. Menopause transition promotes distinct modulation of mRNAs and miRNAs expression in calvaria and bone marrow osteoblastic cells. Cell Biology International, v. 42, n. 1, p. 12-24, JAN 2018. (12/17747-9, 12/15889-0)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

SEMEGHINI, Mayara Sgarbi. Large scale gene expression evaluation of osteoblastic cells from calvaria and bone marrow of ovariectomized rats. 2015. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Odontologia de Ribeirão Preto (PCARP/BC) Ribeirão Preto.