Cytokine profile changes in gingival crevicular fluid after placement different brackets types

Author(s)

Nazar Bergamo, Ana Zilda ^[1] ; Nelson-Filho, Paulo ^[1] ; do Nascimento, Cassio ^[2] ; Viana Casarin, Renato Correa ^[3] ; Casati, Marcio Zaffalon ^[3] ; Damido Andrucioli, Marcela Cristina ^[1] ; Kuchler, Erika Calvano ^[1] ; Longo, Daniele Lucca ^[1] ; Bezerra da Silva, Lea Assed ^[1] ; Nakane Matsumoto, Mirian Aiko ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Pediat Clin, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Sch Dent Ribeirao Preto, Dept Dent Mat & Prosthodont, Ribeirao Preto, SP - Brazil

^[3] Univ Paulista, Sch Dent, Div Periodont, Sao Paulo - Brazil

• Document type: Journal article
• Source: ARCHIVES OF ORAL BIOLOGY; v. 85, p. 79-83, JAN 2018.
• Web of Science Citations: 3

Abstract

Objective: The aim of this study was to examine the relationship between bracket design and ratio of five proinflammatory cytokine, in gingival crevicular fluid (GCF), and bacterial adhesion without tooth movement influence. Design: The sample was comprised of 20 participants, aged 11 to 15 years old (mean age: 13.3 years +/- 1.03). A conventional Gemini (TM) metallic bracket and two self-ligating brackets, In-Ovatiod (R) R and SmartClip (TM), were bonded to the maxillary incisors and canines. GCF was collected using a standard filter paper strip before and 60 days after bonding. The cytokine levels (IL-12, IL-1 alpha, IL-1 beta, IL-6 and TNF-alpha) were performed by the LUMINEX assay. The levels of the red and orange bacterial complexes were analyzed by the Checkerboard DNA-DNA hybridization. The data of cytokine and bacterial complexes were carried out using the non-parametric tests at 5% of significance level. Results: Increased cytokine levels were observed. However, only the SmartClip (TM) group showed a significantly increased level of TNF-alpha (p = 0.046). The SmartClip (TM) brackets group presented higher levels of red complex bacteria. Conclusions: The bracket design affected cytokine levels and bacterial adhesion since it was observed that the proinflammatory cytokines released in GCF to the SmartClip (TM) group showed an increase in the TNF-alpha levels associated with higher bacterial levels, which possibly represents greater inflammatory potential. Thereby, the bracket design should be considered in patients with risk of periodontal disease and root resorption. (AU)

• FAPESP's process: 10/16757-5 - Detection of microorganisms in the saliva and self-ligating brackets by the checkerboard DNA-DNA hybridization: in vivo
• Grantee: Mírian Aiko Nakane Matsumoto
• Support Opportunities: Regular Research Grants