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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

mus-52 disruption and metabolic regulation in Neurospora crassa: Transcriptional responses to extracellular phosphate availability

Full text
Author(s):
Martins, Maira P. [1] ; Gomes, Eriston V. [1] ; Sanches, Pablo R. [1] ; Pedersoli, Wellington R. [1] ; Martinez-Rossi, Nilce M. [1] ; Rossi, Antonio [1]
Total Authors: 6
Affiliation:
[1] Univ Sao Paulo, Ribeirao Preto Med Sch, Dept Genet, Ribeirao Preto, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: PLoS One; v. 13, n. 4 APR 18 2018.
Web of Science Citations: 1
Abstract

Advances in the understanding of molecular systems depend on specific tools like the disruption of genes to produce strains with the desired characteristics. The disruption of any mutagen sensitive (mus) genes in the model fungus Neurospora crassa, i.e. mus-51, mus-52, or mus-53, orthologous to the human genes KU70, KU80, and LIG4, respectively, provides efficient tools for gene targeting. Accordingly, we used RNA-sequencing and reverse transcription-quantitative polymerase chain reaction amplification techniques to evaluate the effects of mus-52 deletion in N. crassa gene transcriptional modulation, and thus, infer its influence regarding metabolic response to extracellular availability of inorganic phosphate (Pi). Notably, the absence of MUS-52 affected the transcription of a vast number of genes, highlighting the expression of those coding for transcription factors, kinases, circadian clocks, oxi-reduction balance, and membrane- and nucleolus-related proteins. These findings may provide insights toward the KU molecular mechanisms, which have been related to telomere maintenance, apoptosis, DNA replication, and gene transcription regulation, as well as associated human conditions including immune system disorders, cancer, and aging. (AU)

FAPESP's process: 16/04274-6 - Transcription factors and cell signaling
Grantee:Eriston Vieira Gomes
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 14/03847-7 - Molecular characterization of mechanisms involved in pathogenicity and cell signaling in fungi
Grantee:Nilce Maria Martinez-Rossi
Support Opportunities: Research Projects - Thematic Grants