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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Monitoring H2O2 inside Aspergillus fumigatus with an Integrated Microelectrode: The Role of Peroxiredoxin Protein Prx1

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Author(s):
Santos, Carla Santana [1] ; Bannitz-Fernandes, Renata [2] ; Lima, Alex S. [1] ; Tairum, Carlos A. [2] ; Malavazi, Iran [3] ; Netto, Luis E. S. [2] ; Bertotti, Mauro [1]
Total Authors: 7
Affiliation:
[1] Univ Sao Paulo, Inst Chem, Dept Fundamental Chem, Av Prof Lineu Prestes 748, BR-05513970 Sao Paulo, SP - Brazil
[2] Univ Sao Paulo, Inst Biosci, Dept Genet & Evolutionary Biol, Rua Matao 321, BR-05508090 Sao Paulo, SP - Brazil
[3] Univ Fed Sao Carlos, Dept Genet & Evolut, Rodovia Washington Luis S-N, BR-13565905 Sao Carlos, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: Analytical Chemistry; v. 90, n. 4, p. 2587-2593, FEB 20 2018.
Web of Science Citations: 3
Abstract

Peroxiredoxins (Prx) are important proteins involved in hydroperoxide degradation and are related to virulence in several pathogens, including Aspergillus fumigatus. In this work, in vivo studies on the degradation of hydrogen peroxide (H2O2) in the microenvironment of A. fumigatus fungus were performed by using an integrated Pt microelectrode. Three A. fumigatus strains were used to confirm the role of the cytosolic protein Prx1 in the defense mechanism of this microorganism: a wild type strain, capable to expressing the protein Prx1; a Delta prx strain, whose gene prx1 was removed; and a genetically complemented Delta prx1::prx1(+) strain generated from the Delta prx1 and in which the gene prx1 was reintroduced. The fabricated microelectrode was shown to be a reliable inert probe tip for in situ and real-time measurements of H2O2 in such microenvironments, with potential applications in investigations involving the mechanism of oxidative stress. (AU)

FAPESP's process: 13/14955-2 - Functional and structural characterization of AfPrxA, a thiol peroxidase from the human pathogen fungus Aspergillus fumigatus and a potential target for drug development
Grantee:Renata Bannitz Fernandes
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)
FAPESP's process: 15/02095-4 - Fabrication of nanosensors to be used in scanning electrochemical microscopy and further application in biological systems (single-cell analysis)
Grantee:Alex da Silva Lima
Support Opportunities: Scholarships in Brazil - Post-Doctoral
FAPESP's process: 15/20776-9 - Fabrication of nanometric probes for the study of biochemical processes by using Scanning Electrochemical Microscopy
Grantee:Mauro Bertotti
Support Opportunities: Regular Research Grants
FAPESP's process: 14/22396-6 - Development of a microsensor coupled to scanning electrochemical microscopy to investigate the role of mitochondria on the respiration process
Grantee:Carla Santana Santos
Support Opportunities: Scholarships in Brazil - Doctorate (Direct)