Nanoparticle-Cell Interactions: Surface Chemistry Effects on the Cellular Uptake of Biocompatible Block Copolymer Assemblies

Author(s)

de Castro, Carlos E. ^[1] ; Ribeiro, Caroline A. S. ^[1] ; Alavarse, Alex C. ^[1] ; Albuquerque, Lindomar J. C. ^[1] ; da Silva, Maria C. C. ^[1] ; Jaeger, Eliezer ^[2] ; Surman, Frantisek ^[2] ; Schmidt, Vanessa ^[3] ; Giacomelli, Cristiano ^[3] ; Giacomelli, Fernando C. ^[1]

Affiliation

^[1] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre - Brazil

^[2] Acad Sci Czech Republ, Inst Macromol Chem, Vvi, Heyrovsky Sq 2, Prague 16206 6 - Czech Republic

^[3] Univ Fed Santa Maria, Dept Quim, BR-97105900 Santa Maria, RS - Brazil

• Document type: Journal article
• Source: Langmuir; v. 34, n. 5, p. 2180-2188, FEB 6 2018.
• Web of Science Citations: 9

Abstract

The development of nanovehicles for intracellular drug delivery is strongly bound to the understating and control of nanoparticles cellular uptake process, which in turn is governed by surface chemistry. In this study, we explored the synthesis, characterization, and cellular uptake of block copolymer assemblies consisting of a pH-responsive poly{[}2-(diisopropyl-amino)ethyl methacrylate] (PDPA) core stabilized by three different biocompatible hydrophilic shells (a zwitterionic type poly(2-methacryloyloxyethyl phosphorylcholine) (PMPC) layer, a highly hydrated poly(ethylene oxide) (PEO) layer with stealth effect, and an also proven nontoxic and nonimmunogenic poly(N-(2-hydroxypropyl)methacrylamide) (PHPMA) layer). All particles had a spherical core-shell structure. The largest particles with the thickest hydrophilic stabilizing shell obtained from PMPC40-b-PDPA(70) were internalized to a higher level than those smaller in size and stabilized by PEO or PHPMA and produced from PEO122-b-PDPA(43) or PHPMA(64)-b-PDPA(72), respectively. Such a behavior was confirmed among different cell lines, with assemblies being internalized to a higher degree in cancer (HeLa) as compared to healthy (Telo-RF) cells. This fact was mainly attributed to the stronger binding of PMPC to cell membranes. Therefore, cellular uptake of nanoparticles at the sub-100 nm size range may be chiefly governed by the chemical nature of the stabilizing layer rather than particles size and/or shell thickness. (AU)

• FAPESP's process: 15/24686-4 - THE INFLUENCE OF SURFACE CHEMISTRY AND PROTEIN CORONA IN THE CELLULAR UPTAKE OF NON-TARGETED POLYMERIC NANOPARTICLES
• Grantee: Carlos Eduardo de Castro
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 17/00459-4 - Biophysicochemical interactions at the nanobiointerface: the effect of surface features and protein corona on the cellular uptake of polymeric assemblies
• Grantee: Fernando Carlos Giacomelli
• Support Opportunities: Regular Research Grants