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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Approaches to convert Mucor circinelloides lipid into biodiesel by enzymatic synthesis assisted by microwave irradiations

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Author(s):
Carvalho, Ana Karine F. [1] ; Bento, Heitor B. S. [1] ; Izario Filho, Held J. [1] ; de Castro, Heizir F. [1]
Total Authors: 4
Affiliation:
[1] Univ Sao Paulo, Engn Sch Lorena, Estr Municipal Campinho S-Nm, BR-12602810 Sao Paulo - Brazil
Total Affiliations: 1
Document type: Journal article
Source: RENEWABLE ENERGY; v. 125, p. 747-754, SEP 2018.
Web of Science Citations: 7
Abstract

This work aimed to study the enzymatic production of biodiesel from lipids produced by Mucor circinelloides URM 4182 using Novozym 435 as catalyst under different conditions, in which microwave irradiations were used to perform both the lipids extraction and transesterification heating. Sequential or simultaneous approaches were also investigated. In direct transesterification, ethanol serves as a solvent for lipid extraction and a reactant for the reaction, simultaneously. Cell growth and the lipids accumulation were carried out in a bioreactor containing glucose and corn steep liquor as carbon and nutrients sources, respectively. Corn steep liquor (CSL) can be a good replacement for synthetic nutrients allowing higher volumetric biomass production (3.1 +/- 0.01 gL(-1)day(-1)). M. circinelloides oil revealed as main fatty acids: palmitic acid C16:0 (18.9%), oleic C18:1 (25.3%), linoleic C18:2 (19.3%) and linoleic acid C18:3 (18.3%). With regards to biodiesel synthesis, the two steps technology, which combined the lipids extraction from microbial biomass followed by enzymatic transesterification reaction both carried out under microwave irradiation, allowing for the attainment of 98.5% ethyl esters in 10 h, compared to 30 h under conventional heating. The simultaneous extraction and transesterification also shows promising results, although the ester content was slight lower (90.1%). (C) 2018 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 16/10636-8 - From the cell factory to the Biodiesel-Bioethanol integrated biorefinery: a systems approach applied to complex problems in micro and macroscales
Grantee:Roberto de Campos Giordano
Support Opportunities: Program for Research on Bioenergy (BIOEN) - Thematic Grants