Identification and Antioxidant Activity of the Extracts of Eugenia uniflora Leaves. Characterization of the Anti-Inflammatory Properties of Aqueous Extract on Diabetes Expression in an Experimental Model of Spontaneous Type 1 Diabetes (NOD Mice)

Author(s)

Gonzalez Schumacher, Nayara Simon ^[1] ; Colomeu, Talita Cristina ^[1] ; de Figueiredo, Daniella ^[1] ; Carvalho, Virginia de Campos ^[1] ; Betim Cazarin, Cinthia Bau ^[2] ; Prado, Marcelo Alexandre ^[2] ; Molina Meletti, Laura Maria ^[3] ; Zollner, Ricardo de Lima ^{[4, 1]}

Affiliation

^[1] Univ Estadual Campinas, Fac Med Sci, Lab Translat Immunol, Rua Vital Brazil, 300, Cidade Univ Zeferino Vaz, BR-13083888 Campinas, SP - Brazil

^[2] Univ Campinas FEA Unicamp, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil

^[3] Agron Inst Campinas, BR-13012970 Campinas, SP - Brazil

^[4] Univ Estadual Campinas, Fac Med Sci, Dept Internal Med, BR-13083887 Campinas, SP - Brazil

• Document type: Journal article
• Source: ANTIOXIDANTS; v. 4, n. 4, p. 662-680, DEC 2015.
• Web of Science Citations: 15

Abstract

Medical and folklore reports suggest that Eugenia uniflora (E. uniflora) is a functional food that contains numerous compounds in its composition, with anti-inflammatory, antioxidant and anti-diabetic effects. In the present study, we investigated the best solvents (water, ethanol and methanol/acetone) for extracting bioactive compounds of E. uniflora leaves, assessing total phenols and the antioxidant activity of the extracts by 2,2-Diphenyl-1-picrylhydrazyl (DPPH), Ferric Reducing Antioxidant Power (FRAP), 2,2 `-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and Oxygen Radical Absorbance Capacity (ORAC) assays, identifying hydrolysable tannins and three phenolic compounds (ellagic acid, gallic acid and rutin) present in the leaves. In addition, we evaluated the incidence of diabetes, degree of insulitis, serum insulin, hepatic glutathione and tolerance test glucose in non-obese diabetic (NOD) mice. Our results suggest that the aqueous extract presents antioxidant activity and high total phenols, which were used as a type 1 diabetes mellitus (DM-1) treatment in NOD mice. We verified that the chronic consumption of aqueous extract reduces the inflammatory infiltrate index in pancreatic islets, maintaining serum insulin levels and hepatic glutathione, and reducing serum lipid peroxidation as well as the risk for diabetes. (AU)

• FAPESP's process: 11/06559-4 - Identification and Characterization of compounds of the aqueous extract of Passiflora alata. Effect of treatment on diabetes expression and modulation of inflammation in pancreatic islets in the NOD mouse model of type 1 diabetes (non-obese diabetic)
• Grantee: Ricardo de Lima Zollner
• Support Opportunities: Regular Research Grants