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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Canine leishmaniasis: Genome-wide analysis and antibody response to Lutzomyia longipalpis saliva

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Batista, Luis F. S. [1, 2] ; Utsunomiya, Yuri T. [3] ; Silva, Thais B. F. [4] ; Carneiro, Mariana M. [1] ; Paiva, Joyr S. F. [1] ; Silva, Rafaela B. [1] ; Tomokane, Thaise Y. [4] ; Rossi, Claudio N. [5] ; Pacheco, Acacio D. [6] ; Torrecilha, Rafaela B. P. [3] ; Silveira, Fernando T. [7] ; Marcondes, Mary [6] ; Nunes, Caris M. [8] ; Laurenti, Marcia D. [4]
Total Authors: 14
[1] Univ Salvador, Escola Saude, Salvador, Ba - Brazil
[2] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Patol Vet, Sao Paulo - Brazil
[3] Univ Estadual Paulista, Fac Ciencias Agr & Vet, Dept Med Vet Prevent & Reprod Anim, Jaboticabal, SP - Brazil
[4] Univ Sao Paulo, Fac Med, Lab Patol Doencas Infecciosas, Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Med Vet & Zootecnia, Dept Clin, Sao Paulo - Brazil
[6] Univ Estadual Paulista, Fac Med Vet, Dept Clin Cirurgia & Reprod Anim, Aracatuba, SP - Brazil
[7] Inst Evandro Chagas, Dept Parasitol, Ananindeua, Para - Brazil
[8] Univ Estadual Paulista, Fac Med Vet, Dept Saude Anim & Prod, Aracatuba, SP - Brazil
Total Affiliations: 8
Document type: Journal article
Source: PLoS One; v. 13, n. 5 MAY 9 2018.
Web of Science Citations: 0

The anti-inflammatory properties of sand fly saliva favor the establishment of the Leishmania infantum infection. In contrast, an antibody response against Lutzomyia longipalpis saliva is often associated with a protective cell-mediated response against canine visceral leishmaniasis. Genetic studies may demonstrate to what extent the ability to secrete antisaliva antibodies depends on genetic or environmental factors. However, the genetic basis of canine antibody response against sand fly saliva has not been assessed. The aim of this study was to identify chromosomal regions associated with the anti-Lu. longipalpis salivary IgG response in 189 dogs resident in endemic areas in order to provide information for prophylactic strategies. Dogs were classified into five groups based on serological and parasitological diagnosis and clinical evaluation. Anti-salivary gland homogenate (SGH) IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). Genomic DNA was isolated from blood samples and genotyped using a SNP chip with 173,662 single nucleotide polymorphism (SNP) markers. The following linear regression model was fitted: IgG level = mean + origin + sex + age + use of a repellent collar, and the residuals were assumed as pseudo-phenotypes for the association test between phenotypes and genotypes (GWA). A component of variance model that takes into account polygenic and sample structure effects (EMMAX) was employed for GWA. Phenotypic findings indicated that anti-SGH IgG levels remained higher in exposed and subclinically infected dogs than in severely diseased dogs even in regression model residuals. Five associated markers were identified on chromosomes 2, 20 and 31. The mapped genes included CD180 (RP105) and MITF related to the rapid activation of B lymphocytes and differentiation into antibody-secreting plasma cells. The findings pointed to chromosomal segments useful for functional confirmation studies and a search for adjuvant molecules of the anti-saliva response. (AU)

FAPESP's process: 12/05847-9 - Clinical, immunological evaluation and their correlation with genetic markers in canine visceral leishmaniasis
Grantee:Luís Fábio da Silva Batista
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 14/01095-8 - Use of next generation sequencing for the fine mapping of loci explaining variance in scrotal circumference in Nellore cattle
Grantee:Yuri Tani Utsunomiya
Support type: Scholarships in Brazil - Doctorate
FAPESP's process: 12/50285-9 - Clinical, immunological features and its correlation with genetic markers in canine visceral leishmaniasis
Grantee:Marcia Dalastra Laurenti
Support type: Regular Research Grants