A non-naturally-occurring P(3HB-co-3HA(MCL)) is produced by recombinant Pseudomonas sp from an unrelated carbon source

Author(s)

Cespedes, Lucas G. ^[1] ; Nahat, Rafael A. T. P. S. ^[1] ; Mendonca, Thatiane T. ^[1] ; Tavares, Rafaela R. ^[1] ; Oliveira-Filho, Edmar R. ^[1] ; Silva, Luiziana F. ^[1] ; Taciro, Marilda K. ^[1] ; Sanchez, Ruben J. ^[2] ; Gomez, Jose G. C. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Av Prof Lineu Prestes 1374 Lab 172, Cidade Univ, BR-05508888 Sao Paulo, SP - Brazil

^[2] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol, Campos Dos Goytacazes, RJ - Brazil

• Document type: Journal article
• Source: International Journal of Biological Macromolecules; v. 114, p. 512-519, JUL 15 2018.
• Web of Science Citations: 2

Abstract

Pseudomonas sp. PHA(-) was used as host for PHA biosynthesis genes from Aeromonas sp. to produce 3HB-co-3HA(MCL) from glucose with no supply of co-substrates. A non-naturally-occurring PHA composed mainly of 3HB, 3HHx and 3HD (3HO,3HDd Delta(5) and 3HDd monomers were detected in smaller amounts) was obtained. The polymer was extracted using two different solvents (acetone and chloroform) and subject to the following characterization tests: FTIR, DSC, TGA and GPC The latter suggests a block copolymer since a single and narrow elution peak was observed for each sample. The DSC results ruled out the possibility of a random copolymer and agrees with a single copolymer composed of two blocks: one with the typical composition of PHA(MCL) produced by Pseudomonas and another containing 3HB and 3HHx with a high 3HHx molar fraction. Thus, this study increases the perspectives of P(3HB-co-3HA(MCL)) production from carbohydrates as the sole carbon source. (C) 2018 Elsevier B.V. All rights reserved. (AU)

• FAPESP's process: 10/51692-1 - Metabolic engineering applied to biopolymer production
• Grantee: Marilda Keico Taciro
• Support Opportunities: Program for Research on Bioenergy (BIOEN) - Young Investigators Grants
• FAPESP's process: 14/15448-0 - Construction of recombinant pseudomonas strains harboring different PHA synthases in its genome to produce 3HB-co-3HAMCL
• Grantee: Edmar Ramos de Oliveira Filho
• Support Opportunities: Scholarships in Brazil - Master