Biochemical and morphological biomarkers of the liver damage in the Neotropical fish, Piaractus mesopotamicus, injected with crude extract of cyanobacterium Radiocystis fernandoi

Author(s)

Tavares, Driele ^[1] ; Paulino, Marcelo Gustavo ^{[1, 2]} ; Terezan, Ana Paula ^[3] ; Fernandes, Joao Batista ^[3] ; Giani, Alessandra ^[4] ; Fernandes, Marisa Narciso ^{[1, 5]}

Affiliation

^[1] Univ Fed Sao Carlos, Dept Physiol Sci, POB 676, Via Washington Luiz, Km 235, BR-13565905 Sao Carlos - Brazil

^[2] Fed Univ Tocantins, Campus Araguaina, Avenida Paraguai S-N, BR-77824838 Araguaina, Tocantins - Brazil

^[3] Univ Fed Sao Carlos, Dept Chem, POB 676, Via Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

^[4] Univ Fed Minas Gerais, Dept Bot, Av Antonio Carlos 6627, BR-31270901 Belo Horizonte, MG - Brazil

^[5] Univ Fed Sao Carlos, Dept Physiol Sci, POB 676, Via Washington Luiz, Km 235, BR-13565905 Sao Carlos, SP - Brazil

• Document type: Journal article
• Source: Environmental Science and Pollution Research; v. 25, n. 16, p. 15349-15356, JUN 2018.
• Web of Science Citations: 1

Abstract

Cyanobacterial proliferation in river and lakes is the result of eutrophication. The cyanobacterium Radiocystis fernandoi strain R28 produces mostly two MC variants MC-RR and MC-YR and small amounts of other oligopeptides, but does not produce MC-LR. The present study investigated the hepatotoxic potential of the crude extract of the R. fernandoi strain R28 on the Neotropical fish, Piaractus mesopotamicus, at 3, 6, and 24 h after intraperitoneal injection (100 mu g MC-LR equivalent per kg(-1) body mass) using biochemical and morphological biomarkers of liver damage. Although the protein phosphatases PP1 and PP2A were not inhibited during the 24-h treatment, liver parenchyma and hepatocyte structure were disrupted. Alkaline phosphatase increased at 3 h post-injection and decreased after 24 h; alanine aminotransferase and aspartate aminotransferase increased in a time-dependent manner up to 24 h indicating impaired liver function. Progressive histopathological changes were consistent with biochemical results demonstrating alterations in liver structure and function. In conclusion, the crude extract of R. fernandoi strain R28 has high hepatotoxic potential and can severely compromise fish health. (AU)

• FAPESP's process: 12/00728-1 - Effects of microcystins present in the crude extract of cyanobacterium, Microcystis aeruginosa, in the liver and muscle of traírão, Hoplias lacerdae.
• Grantee: Marcelo Gustavo Paulino
• Support Opportunities: Scholarships in Brazil - Doctorate