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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Landscape of Transposable Elements Focusing on the B Chromosome of the Cichlid Fish Astatotilapia latifasciata

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Author(s):
Coan, Rafael L. B. [1] ; Martins, Cesar [1]
Total Authors: 2
Affiliation:
[1] Sao Paulo State Univ, UNESP, Dept Morphol, Inst Biosci, BR-18618689 Botucatu, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: GENES; v. 9, n. 6 JUN 2018.
Web of Science Citations: 8
Abstract

B chromosomes (Bs) are supernumerary elements found in many taxonomic groups. Most B chromosomes are rich in heterochromatin and composed of abundant repetitive sequences, especially transposable elements (TEs). B origin is generally linked to the A-chromosome complement (A). The first report of a B chromosome in African cichlids was in Astatotilapia latifasciata, which can harbor 0, 1, or 2 Bs Classical cytogenetic studies found high a TE content on this B chromosome. In this study, we aimed to understand TE composition and expression in the A. latifasciata genome and its relation to the B chromosome. We used bioinformatics analysis to explore the genomic organization of TEs and their composition on the B chromosome. The bioinformatics findings were validated by fluorescent in situ hybridization (FISH) and real-time PCR (qPCR). A. latifasciata has a TE content similar to that of other cichlid fishes and several expanded elements on its B chromosome. With RNA sequencing data (RNA-seq), we showed that all major TE classes are transcribed in the brain, muscle, and male and female gonads. An evaluation of TE transcription levels between B- and B+ individuals showed that few elements are differentially expressed between these groups and that the expanded B elements are not highly transcribed. Putative silencing mechanisms may act on the B chromosome of A. latifasciata to prevent the adverse consequences of repeat transcription and mobilization in the genome. (AU)

FAPESP's process: 14/16763-6 - High scale genomic analysis of repetitive elements focusing on B chromosomes of Astatotilapia latifasciata cichlid
Grantee:Rafael Luiz Buogo Coan
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 15/16661-1 - Sex and B chromosome enigmas: model systems for the study of chromosome and genome evolution
Grantee:César Martins
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 13/04533-3 - Functional analysis of B chromosomes: the cichlid fish Astatotilapia latifasciata as model
Grantee:César Martins
Support Opportunities: Regular Research Grants